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Type: Journal article
Title: PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20
Author: Nicholson, I.
Mavrangelos, C.
Bird, D.
Bresatz-Atkins, S.
Eastaff-Leung, N.
Grose, R.
Gundsambuu, B.
Hill, D.
Millard, D.
Sadlon, T.
To, S.
Zola, H.
Barry, S.
Krumbiegel, D.
Citation: Cellular Immunology, 2012; 275(1):12-18
Publisher: Academic Press Inc
Issue Date: 2012
ISSN: 0008-8749
Statement of
Ian C. Nicholson, Christos Mavrangelos, Daniel R.G. Bird, Suzanne Bresatz-Atkins, Nicola G. Eastaff-Leung, Randall H. Grose, Batjargal Gundsambuu, Danika Hill, Debbrah J. Millard, Timothy J. Sadlon, Sarah To, Heddy Zola, Simon C. Barry, Doreen Krumbiegel
Abstract: The peptidase inhibitor PI16 was shown previously by microarray analysis to be over-expressed by CD4-positive/CD25-positive Treg compared with CD4-positive/CD25-negative Th cells. Using a monoclonal antibody to the human PI16 protein, we found that PI16-positive Treg have a memory (CD45RO-positive) phenotype and express higher levels of FOXP3 than PI16-negative Treg. PI16-positive Treg are functional in suppressor assays in vitro with potency similar to PI16-negative Treg. Further phenotyping of the PI16-positive Treg revealed that the chemokine receptors CCR4 and CCR6 are expressed by more of the PI16-positive/CD45RO-positive Treg compared with PI16-negative/CD45RO-positive Treg or Th cells. PI16-positive Treg showed enhanced in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation. We conclude that PI16 identifies a novel distinct subset of functional memory Treg which can migrate to sites of inflammation and regulate the pro-inflammatory response at those sites.
Keywords: Regulatory T cells; Lymphocyte migration; Memory Treg; Peptidase inhibitor 16
Rights: Copyright © 2012 Elsevier Inc. All rights reserved.
RMID: 0020118988
DOI: 10.1016/j.cellimm.2012.04.002
Appears in Collections:Paediatrics publications

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