Purification and characterization of alkaline pectin lyase from a newly isolated Bacillus clausii and its application in elicitation of plant disease resistance
Date
2012
Authors
Li, Z.
Bai, Z.
Zhang, B.
Li, B.
Jin, B.
Zhang, M.
Lin, F.
Zhang, H.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Applied Biochemistry and Biotechnology, 2012; 167(8):2241-2256
Statement of Responsibility
Zuming Li, Zhihui Bai, Baoguo Zhang, Baojv Li, Bo Jin, Michael Zhang, Francis Lin and Hongxun Zhang
Conference Name
Abstract
Alkaline pectin lyase (PNL) shows potential as a biological control agent against several plant diseases. We isolated and characterized a new Bacillus clausii strain that can produce 4,180 U/g of PNL using sugar beet pulp as a carbon source and inducer. The PNL was purified to apparent homogeneity using ultrafiltration, ammonium sulfate fractionation, DEAE Sepharose Fast Flow, and Sephadex G-75 gel filtration. The purified PNL was found to be a monomeric protein with a molecular weight of 35 kDa, as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). It demonstrated optimal activity with K m of 0.87 mg/ml at pH 10.0 and 60 °C. The enzyme is stable in the pH range of 8.0–10.0 and temperature ≤40 °C. Ca2+ was found to stimulate the enzymatic activity of the PNL by up to 410 %. Mass spectrometric results gave 38 % match coverage with pectate lyase from B. clausii KSM-K16 (gi|56961845). The PNL was found to elicit disease resistance in cucumber seedlings, suggesting that it may have applications in biocontrol and sustainable agriculture.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
© Springer Science+Business Media, LLC 2012