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https://hdl.handle.net/2440/76868
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Type: | Journal article |
Title: | Absence of platelet endothelial cell adhesion molecule-1 (CD31) leads to increased severity of local and systemic IgE-mediated anaphylaxis and modulation of mast cell activation |
Author: | Wong, M.X. Roberts, D. Bartley, P. Jackson, D. |
Citation: | Journal of Immunology, 2002; 168(12):6455-6462 |
Publisher: | American Association of Immunologists |
Issue Date: | 2002 |
ISSN: | 0022-1767 1550-6606 |
Statement of Responsibility: | Mae-Xhum Wong, Donna Roberts, Paul A. Bartley, and Denise E. Jackson |
Abstract: | Platelet endothelial cell adhesion molecule-1 (PECAM-1) is a newly assigned member of the Ig-immunoreceptor tyrosine-based inhibitory motif superfamily, and its functional role is suggested to be an inhibitory receptor that modulates immunoreceptor tyrosine-based activation motif-dependent signaling cascades. In this study, we hypothesized that PECAM-1 plays an essential in vivo role as a counterregulator of immediate hypersensitivity reactions. We found that PECAM-1 was highly expressed on the surface of immature bone marrow mast cells and at a lower density on mature peritoneal mast cells. Examination of skin biopsies from PECAM-1(+/+) and PECAM-1(-/-) mice revealed that absence of PECAM-1 did not affect mast cell development or the capacity of mast cells to populate tissues. To examine whether the absence of PECAM-1 would influence immediate hypersensitivity reactions, PECAM-1(+/+) and PECAM-1(-/-) mice were presensitized with anti-DNP mouse IgE and then challenged 20 h later with DNP-BSA or PBS. PECAM-1(-/-) mice exhibited elevated serum histamine concentrations after Ag stimulation compared with PECAM-1(+/+) mice, indicating an increased severity of systemic IgE-mediated anaphylaxis. PECAM-1(-/-) mice have increased sensitivity to local cutaneous IgE-dependent anaphylaxis compared with PECAM-1(+/+) mice, as assessed by greater tissue swelling of their ears and mast cell degranulation in situ. PECAM-1(-/-) bone marrow mast cells showed enhanced dense granule serotonin release after Fc epsilon RI cross-linking in vitro. These results suggest that PECAM-1 acts as a counterregulator in allergic disease susceptibility and severity and negatively modulates mast cell activation. |
Keywords: | Bone Marrow Cells Cells, Cultured Mast Cells Animals Mice, Inbred C57BL Mice, Knockout Mice Anaphylaxis Disease Susceptibility Edema Immunoglobulin E Receptors, IgE Passive Cutaneous Anaphylaxis Severity of Illness Index Cell Differentiation Cell Degranulation Organ Specificity Up-Regulation Platelet Endothelial Cell Adhesion Molecule-1 |
Rights: | Copyright © 2002 by The American Association of Immunologists |
DOI: | 10.4049/jimmunol.168.12.6455 |
Description (link): | http://jimmunol.org/content/168/12/6455 |
Published version: | http://dx.doi.org/10.4049/jimmunol.168.12.6455 |
Appears in Collections: | Aurora harvest Psychology publications |
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