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Type: Journal article
Title: Diversity in phenotype and steroid hormone dependence in dendritic cells and macrophages in the mouse uterus
Author: Hudson Keenihan, S.
Robertson, S.
Citation: Biology of Reproduction, 2004; 70(6):1562-1572
Publisher: Soc Study Reproduction
Issue Date: 2004
ISSN: 0006-3363
Statement of
Sarah N. Hudson Keenihan, and Sarah A. Robertson
Abstract: The dendritic cells and related antigen-presenting cells (APCs) that activate lymphocytes for acquired immunity in the female reproductive tract are not well characterized. The aim of the present study was to examine heterogeneity among uterine APCs in mice and, specifically, to determine whether phenotypically and functionally distinct subpopulations of dendritic cells and macrophages can be identified. Using immunohistochemistry, abundant cells expressing APC-restricted molecules major histocompatibility complex (MHC) class II, F4/80, class A scavenger receptor, macrosialin, and sialoadhesin were evident in estrous mice. Cells expressing the costimulatory molecule B7-2 were rarely observed. Flow cytometric analysis revealed three subpopulations of uterine APCs. Undifferentiated macrophages were F4/80-positive (+), MHC class II-negative (–) cells, of which 70–80% expressed CD11b, but few expressed class A scavenger receptor, macrosialin, or sialoadhesin. Mature macrophages were F4/80+/MHC class II+ cells, of which approximately 50% expressed CD11b, class A scavenger receptor, macrosialin, and sialoadhesin. Uterine dendritic cells were F4/ 80–/MHC class II+ cells, with stimulatory immunoaccessory function relative to uterine macrophages and heterogeneous expression of dendritic markers 33D1, DEC205, CD11c, and CD1. Experiments in ovariectomized mice showed that undifferentiated macrophages were steroid hormone dependent but that mature macrophages and dendritic cells persisted after depletion of ovarian steroid hormones, although with altered phenotypes. In summary, our findings identify three discrete populations of APCs inhabiting the murine uterus and suggest that both mature macrophages and dendritic cells differentiate from undifferentiated macrophage precursor cells. Plasticity in the ontogenetic and functional relationships between uterine dendritic cells and macrophages likely is critical in regulating immune responses conducive to reproductive success.
Keywords: Uterus
Dendritic Cells
Mice, Inbred BALB C
Mice, Inbred C57BL
Membrane Glycoproteins
Receptors, Immunologic
Antigens, Differentiation
Antigens, CD
Antigens, CD1
Antigens, Differentiation, Myelomonocytic
Histocompatibility Antigens Class II
Cell Differentiation
Receptors, Scavenger
Scavenger Receptors, Class A
Sialic Acid Binding Ig-like Lectin 1
CD11b Antigen
Description: © 2004 by the Society for the Study of Reproduction, Inc.
DOI: 10.1095/biolreprod.103.024794
Appears in Collections:Aurora harvest
Obstetrics and Gynaecology publications

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