Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/80621
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Expression of an anti-CD4 single-chain antibody fragment from the donor cornea can prolong corneal allograft survival in inbred rats |
Author: | Appleby, S. Jessup, C. Mortimer, L. Kirk, K. Brereton, H. Coster, D. Tan, C. Williams, K. |
Citation: | British Journal of Ophthalmology, 2013; 97(1):101-105 |
Publisher: | British Med Journal Publ Group |
Issue Date: | 2013 |
ISSN: | 0007-1161 1468-2079 |
Statement of Responsibility: | Sarah Louise Appleby, Claire F Jessup, Lauren A Mortimer, Kirsty Kirk, Helen M Brereton, Douglas John Coster, Chuan K Tan, Keryn A Williams |
Abstract: | <h4>Aim</h4>To investigate whether expression of an anti-CD4 antibody fragment (scFv) by a lentivector-transduced donor cornea can prolong rat corneal allograft survival.<h4>Methods</h4>Inbred Fischer 344 rats received penetrating corneal allografts from Wistar-Furth donors after a 3 h transduction of the donor cornea with a lentivector carrying anti-CD4scFv cDNA (Lv-CD4scFv), a lentivector carrying the reporter gene-enhanced yellow fluorescence protein (LV-eYFP), or an adenoviral vector carrying anti-CD4 scFv cDNA (Ad-CD4scFv). Unmodified controls were also performed. Graft survival was assessed by corneal clarity, and rejection was confirmed histologically.<h4>Results</h4>In organ-cultured corneas, expression of anti-CD4 scFv was detected at 2 days post-transduction with the adenoviral vector, compared with 5 days post-transduction with the lentivector, and was 10-fold higher than the former. More inflammation was observed in Ad-CD4scFv-modified allografts than in Lv-CD4scFv-modified grafts at 15 days postsurgery (p=0.01). The median time to rejection for unmodified, LV-eYFP and Ad-CD4scFv grafts was day 17, compared with day 22 for Lv-CD4scFv grafts (p≤0.018).<h4>Conclusion</h4>Donor corneas transduced with a lentiviral vector carrying anti-CD4scFv cDNA showed a modest but significant prolongation in graft survival compared with unmodified, Lv-eYFP and Ad-CD4scFv grafts. However, rejection still occurred in all Lv-CD4scFv grafts, indicating that sensitisation may have been delayed but was not prevented. |
Keywords: | Cornea CD4-Positive T-Lymphocytes Animals Rats, Inbred F344 Rats, Inbred WF Rats Adenoviridae Bacterial Proteins Luminescent Proteins Fluorescent Dyes Enzyme-Linked Immunosorbent Assay Keratoplasty, Penetrating Transplantation, Homologous Flow Cytometry Transfection Graft Survival Gene Expression Regulation Genes, Reporter Genetic Vectors Tissue Donors Male Single-Chain Antibodies |
Rights: | Copyright status unknown |
DOI: | 10.1136/bjophthalmol-2012-302360 |
Published version: | http://dx.doi.org/10.1136/bjophthalmol-2012-302360 |
Appears in Collections: | Aurora harvest Paediatrics publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.