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Type: Journal article
Title: Differential distribution of mRNA for the α- and β-subunits of chorionic gonadotrophin in the implantation stage blastocyst of the marmoset monkey
Other Titles: Differential distribution of mRNA for the alpha- and beta-subunits of chorionic gonadotrophin in the implantation stage blastocyst of the marmoset monkey
Author: Lopata, A.
Berka, J.
Simula, A.
Norman, R.
Otani, T.
Citation: Placenta, 1995; 16(4):335-346
Publisher: W.B. Saunders
Issue Date: 1995
ISSN: 0143-4004
Statement of
Lopata, A. ; Berka, J. ; Simula, A. ; Norman, R. ; Otani, T.
Abstract: We studied the expression of mRNA encoding the alpha- and beta-subunits of marmoset chorionic gonadotrophin (mCG) in implantation stage blastocysts and in a trophoblastic cell line derived from such blastocysts. In this investigation in situ hybridization was carried out using digoxygenin-labelled riboprobes to localize the subunit transcripts. The trophoblastic cell line, known to secrete bioactive mCG, was used as a positive control. Marmoset uterine embryos were cultured to hatched blastocysts and following growth on Matrigel or plastic were processed for in situ hybridization at developmental stages ranging from 13-15 days post-conception. In serial sections mCG-beta mRNA was detected mainly in polar trophoblast. The mRNA for mCG-alpha was expressed more uniformly in polar and mural trophoblast. Transcripts for the beta-subunit were not expressed, or present as weak signals, in the inner cell mass (ICM) and endoderm. However, low levels of mRNA for mCG-alpha were detected in the ICM and visceral endoderm. We have concluded that mRNA for mCG-beta was primarily localized to patches of syncytiotrophoblast at the embryonic pole and sparsely distributed in mural trophoblast, while the transcripts for mCG-alpha were distributed more uniformly in differentiating cytotrophoblast and syncytium, and at much lower levels in ICM and early endoderm.
Keywords: Cells, Cultured
Cell Line
Chorionic Gonadotropin
RNA Probes
RNA, Messenger
In Situ Hybridization
Transcription, Genetic
Embryonic and Fetal Development
Embryo Implantation
DOI: 10.1016/0143-4004(95)90091-8
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Appears in Collections:Aurora harvest
Obstetrics and Gynaecology publications

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