Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/87422
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Type: Journal article
Title: Glucagon-like peptide 1 attenuates the acceleration of gastric emptying induced by hypoglycemia in healthy subjects
Author: Plummer, M.
Jones, K.
Annink, C.
Cousins, C.
Meier, J.
Chapman, M.
Horowitz, M.
Deane, A.
Citation: Diabetes Care, 2014; 37(6):1509-1515
Publisher: American Diabetes Association
Issue Date: 2014
ISSN: 0149-5992
1935-5548
Statement of
Responsibility: 
Mark P. Plummer, Karen L. Jones, Chris E. Annink, Caroline E. Cousins, Juris J. Meier, Marianne J. Chapman, Michael Horowitz and Adam M. Deane
Abstract: OBJECTIVE: Exogenous GLP-1 slows gastric emptying in health and diabetes leading to diminished glycemic excursions. Gastric emptying is markedly accelerated by hypoglycemia. The primary objective was to determine whether GLP-1 attenuates the acceleration of gastric emptying induced by hypoglycemia. RESEARCH DESIGN AND METHODS: Ten healthy volunteers were studied on four separate days in a randomized double-blind fashion. Blood glucose was stabilized using a glucose/insulin clamp at hypoglycemia (2.6 mmol/L on two occasions [hypo]) or euglycemia (6.0 mmol/L on two occasions [eu]) between T = -15 and 45 min before clamping at 6.0 mmol/L until 180 min. During hypoglycemia and euglycemia, subjects received intravenous GLP-1 (1.2 pmol/kg/min) or placebo. At T = 0 min, subjects ingested 100 g beef mince labeled with 20 MBq (99m)Tc-sulfur-colloid and 3 g of 3-O-methyl-glucose (3-OMG), a marker of glucose absorption. Gastric emptying was measured scintigraphically from T = 0 to 180 min and serum 3-OMG taken at 15-min intervals. The areas under the curve for gastric emptying and 3-OMG concentration were analyzed using one-way repeated-measures ANOVA with Bonferroni-Holm adjusted post hoc tests. RESULTS: Gastric emptying was accelerated during hypoglycemia (hypo/placebo vs. eu/placebo; P < 0.001), as was glucose absorption (P < 0.03). GLP-1 slowed emptying during euglycemia (eu/placebo vs. eu/GLP-1; P < 0.001). However, hypoglycemia-induced acceleration of gastric emptying on placebo was markedly diminished by GLP-1 (hypo/placebo vs. hypo/GLP-1; P < 0.008), as was glucose absorption (P < 0.01). CONCLUSIONS: Acute administration of exogenous GLP-1 attenuates, but does not abolish, the acceleration of gastric emptying by insulin-induced hypoglycemia in healthy subjects.
Keywords: Humans; Hypoglycemia; Insulin; Blood Glucose; Hypoglycemic Agents; Glucose Clamp Technique; Injections, Intravenous; Double-Blind Method; Gastric Emptying; Aged; Middle Aged; Female; Male; Glucagon-Like Peptide 1; Incretins; Healthy Volunteers
Rights: © 2014 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.
RMID: 0030011080
DOI: 10.2337/dc13-1813
Grant ID: http://purl.org/au-research/grants/nhmrc/1025648
Appears in Collections:Medicine publications

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