Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Soybean SAT1 (Symbiotic Ammonium Transporter 1) encodes a bHLH transcription factor involved in nodule growth and NH₄⁺ transport
Other Titles: Soybean SAT1 (Symbiotic Ammonium Transporter 1) encodes a bHLH transcription factor involved in nodule growth and NH(4)(+) transport
Author: Chiasson, D.
Loughlin, P.
Mazurkiewicz, D.
Mohammadidehcheshmeh, M.
Fedorova, E.
Okamoto, M.
McLean, E.
Glass, A.
Smith, S.
Bisseling, T.
Tyerman, S.
Day, D.
Kaiser, B.
Citation: Proceedings of the National Academy of Sciences of the United States of America, 2014; 111(13):4814-4819
Publisher: National Academy of Sciences
Issue Date: 2014
ISSN: 0027-8424
Statement of
David M. Chiasson, Patrick C. Loughlin, Danielle Mazurkiewicz, Manijeh Mohammadidehcheshmeh, Elena E. Fedorova, Mamoru Okamoto, Elizabeth McLean, Anthony D. M. Glass, Sally E. Smith, Ton Bisseling, Stephen D. Tyerman, David A. Day, and Brent N. Kaiser
Abstract: Glycine max symbiotic ammonium transporter 1 was first documented as a putative ammonium (NH4(+)) channel localized to the symbiosome membrane of soybean root nodules. We show that Glycine max symbiotic ammonium transporter 1 is actually a membrane-localized basic helix-loop-helix (bHLH) DNA-binding transcription factor now renamed Glycine max bHLH membrane 1 (GmbHLHm1). In yeast, GmbHLHm1 enters the nucleus and transcriptionally activates a unique plasma membrane NH4(+) channel Saccharomyces cerevisiae ammonium facilitator 1. Ammonium facilitator 1 homologs are present in soybean and other plant species, where they often share chromosomal microsynteny with bHLHm1 loci. GmbHLHm1 is important to the soybean rhizobium symbiosis because loss of activity results in a reduction of nodule fitness and growth. Transcriptional changes in nodules highlight downstream signaling pathways involving circadian clock regulation, nutrient transport, hormone signaling, and cell wall modification. Collectively, these results show that GmbHLHm1 influences nodule development and activity and is linked to a novel mechanism for NH4(+) transport common to both yeast and plants.
Keywords: Cell Membrane; Saccharomyces cerevisiae; Soybeans; Ammonium Compounds; Cation Transport Proteins; Soybean Proteins; DNA, Plant; Gene Expression Regulation, Plant; Protein Binding; Biological Transport; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; Root Nodules, Plant
RMID: 0030007268
DOI: 10.1073/pnas.1312801111
Grant ID:
Appears in Collections:Agriculture, Food and Wine publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.