Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Evaluation of four endogenous reference genes and their real-time PCR assays for common wheat quantification in GMOs detection
Author: Huang, H.
Cheng, F.
Wang, R.
Zhang, D.
Yang, L.
Citation: PLoS One, 2013; 8(9):e75850-1-e75850-9
Publisher: Public Library of Science
Issue Date: 2013
ISSN: 1932-6203
Statement of
Huali Huang, Fang Cheng, Ruoan Wang, Dabing Zhang, Litao Yang
Abstract: Proper selection of endogenous reference genes and their real-time PCR assays is quite important in genetically modified organisms (GMOs) detection. To find a suitable endogenous reference gene and its real-time PCR assay for common wheat (Triticum aestivum L.) DNA content or copy number quantification, four previously reported wheat endogenous reference genes and their real-time PCR assays were comprehensively evaluated for the target gene sequence variation and their real-time PCR performance among 37 common wheat lines. Three SNPs were observed in the PKABA1 and ALMT1 genes, and these SNPs significantly decreased the efficiency of real-time PCR amplification. GeNorm analysis of the real-time PCR performance of each gene among common wheat lines showed that the Waxy-D1 assay had the lowest M values with the best stability among all tested lines. All results indicated that the Waxy-D1 gene and its real-time PCR assay were most suitable to be used as an endogenous reference gene for common wheat DNA content quantification. The validated Waxy-D1 gene assay will be useful in establishing accurate and creditable qualitative and quantitative PCR analysis of GM wheat.
Keywords: Plants, Genetically Modified; Triticum; Starch Synthase; Plant Proteins; DNA Primers; Reproducibility of Results; Polymorphism, Single Nucleotide; Genes, Plant; Genetic Variation; Real-Time Polymerase Chain Reaction
Rights: © 2013 Huang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
RMID: 0030023452
DOI: 10.1371/journal.pone.0075850
Appears in Collections:Agriculture, Food and Wine publications

Files in This Item:
File Description SizeFormat 
hdl_89632.pdfPublished version733.75 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.