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Type: Journal article
Title: Dietary protein intake and stage of lactation differentially modulate amino acid transporter mRNA abundance in porcine mammary tissue
Author: Laspiur, J.
Burton, J.
Weber, P.
Moore, J.
Kirkwood, R.
Trottier, N.
Citation: Journal of Nutrition, 2009; 139(9):1677-1684
Publisher: American Society for Nutrition
Issue Date: 2009
ISSN: 0022-3166
Statement of
Juliana Pérez Laspiur, Jeanne L. Burton, Patty S. D. Weber, Julie Moore, Roy N. Kirkwood, and Nathalie L. Trottier
Abstract: To test the hypothesis that under restricted and surfeit protein intake the mammary gland undergoes adaptive regulation, changes in mammary tissue mRNA abundance of cationic amino acid (AA) transporter (CAT)-1, CAT-2B, alanine/serine/cysteine/threonine transporter 1 (ASCT1), and broad specificity transporter for neutral and cationic AA (ATB⁰,⁺), and CAT-1 protein abundance were investigated at 2 stages of lactation. Eighteen sows were allocated to a 2 × 3 randomized incomplete block design with 2 stages of lactation (early and peak) and 3 protein levels: deficient (D), adequate (A), or in excess (E) of lactation requirement. In early lactation, compared with A, sows fed E had lower (P = 0.05) piglet growth rate and sows fed D or E had lower (P ≤ 0.05) casein yield. In early lactation, piglet growth rate and milk protein and casein yield increased from D to A and decreased from A to E (quadratic, P = 0.095, P < 0.05, and P < 0.01, respectively). Protein intake did not affect CAT-1, ASCT1, ATB⁰,⁺ mRNA abundance, or CAT-1 protein level. Overall, CAT-2B mRNA abundance decreased linearly with increasing protein intake (P < 0.05). Compared with A, E decreased CAT-2B mRNA abundance (P < 0.05). Compared with early lactation, peak lactation did not increase CAT-1 mRNA abundance or relative CAT-1 protein content, but increased abundance of ASCT1 and ATB⁰,⁺ mRNA (P < 0.01). Mammary CAT-2B appears to be adaptively regulated in vivo at the transcription level, whereas ASCT1 and ATB⁰,⁺ mRNA abundances are associated only with stage of lactation. Neither protein intake nor stage of lactation affects porcine mammary CAT-1 gene expression in vivo.
Rights: © 2009 American Society for Nutrition.
RMID: 0030006859
DOI: 10.3945/jn.108.103549
Appears in Collections:Animal and Veterinary Sciences publications

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