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https://hdl.handle.net/2440/94010
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Type: | Journal article |
Title: | Dietary protein intake and stage of lactation differentially modulate amino acid transporter mRNA abundance in porcine mammary tissue |
Author: | Laspiur, J. Burton, J. Weber, P. Moore, J. Kirkwood, R. Trottier, N. |
Citation: | The Journal of Nutrition, 2009; 139(9):1677-1684 |
Publisher: | American Society for Nutrition |
Issue Date: | 2009 |
ISSN: | 0022-3166 1541-6100 |
Statement of Responsibility: | Juliana Pérez Laspiur, Jeanne L. Burton, Patty S. D. Weber, Julie Moore, Roy N. Kirkwood, and Nathalie L. Trottier |
Abstract: | To test the hypothesis that under restricted and surfeit protein intake the mammary gland undergoes adaptive regulation, changes in mammary tissue mRNA abundance of cationic amino acid (AA) transporter (CAT)-1, CAT-2B, alanine/serine/cysteine/threonine transporter 1 (ASCT1), and broad specificity transporter for neutral and cationic AA (ATB⁰,⁺), and CAT-1 protein abundance were investigated at 2 stages of lactation. Eighteen sows were allocated to a 2 × 3 randomized incomplete block design with 2 stages of lactation (early and peak) and 3 protein levels: deficient (D), adequate (A), or in excess (E) of lactation requirement. In early lactation, compared with A, sows fed E had lower (P = 0.05) piglet growth rate and sows fed D or E had lower (P ≤ 0.05) casein yield. In early lactation, piglet growth rate and milk protein and casein yield increased from D to A and decreased from A to E (quadratic, P = 0.095, P < 0.05, and P < 0.01, respectively). Protein intake did not affect CAT-1, ASCT1, ATB⁰,⁺ mRNA abundance, or CAT-1 protein level. Overall, CAT-2B mRNA abundance decreased linearly with increasing protein intake (P < 0.05). Compared with A, E decreased CAT-2B mRNA abundance (P < 0.05). Compared with early lactation, peak lactation did not increase CAT-1 mRNA abundance or relative CAT-1 protein content, but increased abundance of ASCT1 and ATB⁰,⁺ mRNA (P < 0.01). Mammary CAT-2B appears to be adaptively regulated in vivo at the transcription level, whereas ASCT1 and ATB⁰,⁺ mRNA abundances are associated only with stage of lactation. Neither protein intake nor stage of lactation affects porcine mammary CAT-1 gene expression in vivo. |
Keywords: | Mammary Glands, Animal Milk Animals Animals, Newborn Swine Protein Deficiency Weight Gain Amino Acids Amino Acid Transport Systems Dietary Proteins Caseins RNA, Messenger Diet Random Allocation Lactation Female |
Rights: | © 2009 American Society for Nutrition. |
DOI: | 10.3945/jn.108.103549 |
Published version: | http://dx.doi.org/10.3945/jn.108.103549 |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest 2 |
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