Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/98015
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Type: | Conference paper |
Title: | Localised hydrogen peroxide sensing for reproductive health |
Author: | Purdey, M. Schartner, E. Sutton-McDowall, M. Ritter, L. Thompson, J. Monro, T. Abell, A. |
Citation: | Proceedings of SPIE, 2015 / Baldini, F., Homola, J., Lieberman, R. (ed./s), vol.9506, pp.950614-1-950614-10 |
Publisher: | SPIE |
Issue Date: | 2015 |
Series/Report no.: | Proceedings of SPIE |
ISBN: | 9781628416275 |
ISSN: | 0277-786X 1996-756X |
Conference Name: | SPIE Optics + Optoelectronics (13 Apr 2015 - 16 Apr 2015 : Prague, Czech Republic) |
Editor: | Baldini, F. Homola, J. Lieberman, R. |
Statement of Responsibility: | Malcolm S Purdey, Erik P Schartner, Melanie L Sutton-McDowall, Lesley J Ritter, Jeremy G Thompson, Tanya M Monro, and Andrew D Abell |
Abstract: | The production of reactive oxygen species (ROS) is known to affect the developmental competence of embryos. Hydrogen peroxide (H₂O₂) an important reactive oxygen species, is also known to causes DNA damage and defective sperm function. Current techniques require incubating a developing embryo with an organic fluorophore which is potentially hazardous for the embryo. What we need is a localised ROS sensor which does not require fluorophores in solution and hence will allow continuous monitoring of H₂O₂ production without adversely affect the development of the embryo. Here we report studies on such a fibre-based sensor for the detection of H₂O₂ that uses a surface-bound aryl boronate fluorophore carboxyperoxyfluor-1(CPF1). Optical fibres present a unique platform due to desirable characteristics as dip sensors in biological solutions. Attempts to functionalise the fibre tips using polyelectrolyte layers and (3-aminopropyl)triethoxysilane (APTES) coatings resulted in a limited signal and poor fluorescent response to H₂O₂ due to a low tip surface density of the fluorophore. To increase the surface density, CPF1 was integrated into a polymer matrix formed on the fibre tip by a UV-catalysed polymerisation process of acrylamide onto a methacrylate silane layer. The polyacrylamide containing CPF1 gave a much higher surface density than previous surface attachment methods and the sensor was found to effectively detect H₂O₂. Using this method, biologically relevant concentrations of H₂O₂ were detected, enabling remote sensing studies into ROS releases from embryos throughout early development. |
Description: | Session 10 - Chemical Sensors and Biosensors II |
Rights: | © 2015 SPIE |
DOI: | 10.1117/12.2178680 |
Published version: | http://dx.doi.org/10.1117/12.2178680 |
Appears in Collections: | Aurora harvest 3 Medicine publications |
Files in This Item:
File | Description | Size | Format | |
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hdl_98015.pdf | Published version | 767.99 kB | Adobe PDF | View/Open |
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