Evidence for the Application of Emerging Technologies to Accelerate Crop Improvement – A Collaborative Pipeline to Introgress Herbicide Tolerance Into Chickpea

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dc.contributor.authorCroser, J.
dc.contributor.authorMao, D.
dc.contributor.authorDron, N.
dc.contributor.authorMichelmore, S.
dc.contributor.authorMcMurray, L.
dc.contributor.authorPreston, C.
dc.contributor.authorBruce, D.
dc.contributor.authorOgbonnaya, F.C.
dc.contributor.authorRibalta, F.M.
dc.contributor.authorHayes, J.
dc.contributor.authorLichtenzveig, J.
dc.contributor.authorErskine, W.
dc.contributor.authorCullis, B.
dc.contributor.authorSutton, T.
dc.contributor.authorHobson, K.
dc.date.issued2021
dc.description.abstractAccelerating genetic gain in crop improvement is required to ensure improved yield and yield stability under increasingly challenging climatic conditions. This case study demonstrates the effective confluence of innovative breeding technologies within a collaborative breeding framework to develop and rapidly introgress imidazolinone Group 2 herbicide tolerance into an adapted Australian chickpea genetic background. A well-adapted, high-yielding desi cultivar PBA HatTrick was treated with ethyl methanesulfonate to generate mutations in the ACETOHYDROXYACID SYNTHASE 1 (CaAHAS1) gene. After 2 years of field screening with imidazolinone herbicide across >20 ha and controlled environment progeny screening, two selections were identified which exhibited putative herbicide tolerance. Both selections contained the same single amino acid substitution, from alanine to valine at position 205 (A₂₀₅V) in the AHAS1 protein, and KASP™ markers were developed to discriminate between tolerant and intolerant genotypes. A pipeline combining conventional crossing and F₂ production with accelerated single seed descent from F₂:₄ and marker-assisted selection at F₂ rapidly introgressed the herbicide tolerance trait from one of the mutant selections, D15PAHI002, into PBA Seamer, a desi cultivar adapted to Australian cropping areas. Field evaluation of the derivatives of the D15PAHI002 PBA Seamer cross was analyzed using a factor analytic mixed model statistical approach designed to accommodate low seed numbers resulting from accelerated single seed descent. To further accelerate trait introgression, field evaluation trials were undertaken concurrent with crop safety testing trials. In 2020, 4 years after the initial cross, an advanced line selection CBA2061, bearing acetohydroxyacid synthase (AHAS) inhibitor tolerance and agronomic and disease resistance traits comparable to parent PBA Seamer, was entered into Australian National Variety Trials as a precursor to cultivar registration. The combination of cross-institutional collaboration and the application of novel prebreeding platforms and statistical technologies facilitated a 3-year saving compared to a traditional breeding approach. This breeding pipeline can be used as a model to accelerate genetic gain in other self-pollinating species, particularly food legumes.
dc.description.statementofresponsibilityJanine Croser, Dili Mao, Nicole Dron, Simon Michelmore, Larn McMurray, Christopher Preston, Dylan Bruce, Francis Chuks Ogbonnaya, Federico Martin Ribalta, Julie Hayes, Judith Lichtenzveig, William Erskine, Brian Cullis, Tim Sutton, and Kristy Hobson
dc.identifier.citationFrontiers in Plant Science, 2021; 12:779122-1-779122-13
dc.identifier.doi10.3389/fpls.2021.779122
dc.identifier.issn1664-462X
dc.identifier.issn1664-462X
dc.identifier.orcidMao, D. [0000-0002-0972-8984]
dc.identifier.orcidMichelmore, S. [0000-0003-1725-2522]
dc.identifier.orcidPreston, C. [0000-0002-7588-124X]
dc.identifier.orcidHayes, J. [0000-0001-8716-5024]
dc.identifier.urihttps://hdl.handle.net/2440/146255
dc.language.isoen
dc.publisherFrontiers Media SA
dc.rights© 2021 Croser, Mao, Dron, Michelmore, McMurray, Preston, Bruce, Ogbonnaya, Ribalta, Hayes, Lichtenzveig, Erskine, Cullis, Sutton and Hobson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.source.urihttps://doi.org/10.3389/fpls.2021.779122
dc.subjectmutation; imidazolinone; sulfonylurea; marker-assisted selection (MAS); accelerated single seed descent; sparse phenotyping
dc.titleEvidence for the Application of Emerging Technologies to Accelerate Crop Improvement – A Collaborative Pipeline to Introgress Herbicide Tolerance Into Chickpea
dc.typeJournal article
pubs.publication-statusPublished

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