Seminal Fluid Interaction with Female Reproductive Tract Epithelial Cells: In Vitro Models for Human

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dc.contributor.authorSharkey, D.J.
dc.contributor.authorLyons, H.E.
dc.contributor.authorChan, H.Y.
dc.contributor.authorRobertson, S.A.
dc.contributor.editorÁlvarez-Rodríguez, M.
dc.date.issued2025
dc.descriptionSeries Editor John M. Walker, Print ISSN 1064-3745 ; Electronic ISSN 1940-6029.
dc.description.abstractSeminal fluid introduced into the female reproductive tract following unprotected coitus initiates an inflammation-like response in the cervical tissues that stimulates immune tolerance to male seminal fluid alloantigens and promotes immune adaptation for pregnancy. This response commences when seminal fluid factors induce genes encoding pro-inflammatory cytokines and chemokines including CSF2, IL6, and CXCL8, that in turn cause recruitment of macrophages, dendritic cells, and T cells into the epithelial and stromal layers. Various signaling agents present in seminal plasma provoke the post-coital pro-inflammatory activation and regulate the quality of immune response. However, the factors identified to date do not account for all the biological activity within seminal fluid, implying sperm-associated factors may also contribute. In this chapter, we report methods for evaluating the effects of human seminal fluid components (seminal plasma and sperm) on the female reproductive tract immune response in primary and immortalized ectocervical epithelial cells in vitro.
dc.description.statementofresponsibilityDavid J. Sharkey, Hannah E. Lyons, Hon Yeung Chan, and Sarah A. Robertson
dc.identifier.citationSpermatology: Methods and Protocols, 2025 / Álvarez-Rodríguez, M. (ed./s), vol.2897, Ch.45, pp.663-673
dc.identifier.doi10.1007/978-1-0716-4406-5_45
dc.identifier.isbn978-1-0716-4406-5
dc.identifier.orcidSharkey, D.J. [0000-0002-4831-7950]
dc.identifier.orcidLyons, H.E. [0000-0001-5305-2654]
dc.identifier.orcidRobertson, S.A. [0000-0002-9967-0084]
dc.identifier.urihttps://hdl.handle.net/2440/148364
dc.language.isoen
dc.publisherHumana Press
dc.publisher.placeNew York, NY, USA
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1041335
dc.relation.ispartofseriesMethods in Molecular Biology; 2897
dc.rights© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025
dc.source.urihttps://doi.org/10.1007/978-1-0716-4406-5
dc.subjectseminal fluid; seminal plasma; sperm; cervix; inflammation; cytokines; human
dc.subject.meshGenitalia, Female
dc.subject.meshCervix Uteri
dc.subject.meshSpermatozoa
dc.subject.meshEpithelial Cells
dc.subject.meshSemen
dc.subject.meshHumans
dc.subject.meshCytokines
dc.subject.meshFemale
dc.subject.meshMale
dc.titleSeminal Fluid Interaction with Female Reproductive Tract Epithelial Cells: In Vitro Models for Human
dc.typeBook chapter
pubs.publication-statusPublished

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