Regulation of tcpgenes in classical and El Tor strains of Vibrio cholerae O1
Date
1995
Authors
Thomas, Sonia
Williams, Susan G.
Manning, Paul Alexander
Editors
Advisors
Journal Title
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Volume Title
Type:
Journal article
Citation
Gene, 1995; 166(1):43-48
Statement of Responsibility
Sonia Thomas, Susan G. Williams and Paul A. Manning
Conference Name
Abstract
Expression of genes encoding the toxin-co-regulated pilus (TCP) varies between the two biotypes of Vibrio cholerae O1. Sequence analysis of the tcp locus from the classical and El Tor strains has revealed differences in the intergenic regions between tcpI and tcpP, and tcpH and tcpA, which may be involved in regulation. To investigate this possibility, transcription of tcpA, and the predicted upstream promoters for tcpI and tcpP, has been analysed in the classical and El Tor strains using promoter-cat (chloramphenicol acetyltransferase) fusions. Together with primer extension analyses, these studies indicate that the tcpA and tcpP promoters are toxR-dependent and suggest that TcpP may be involved in activation of both the tcpI and tcpP promoters. We conclude that differences in the level of tcpA expression in a classical and an El Tor strain are likely to be due to the effect of sequence variation on the ability of control factors to act on these regulatory regions.
School/Discipline
Department of Microbiology and Immunology
Dissertation Note
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© 1995 Elsevier Science B.V. All rights reserved.