Regulation of tcpgenes in classical and El Tor strains of Vibrio cholerae O1

Simple item page
dc.contributor.authorThomas, Soniaen
dc.contributor.authorWilliams, Susan G.en
dc.contributor.authorManning, Paul Alexanderen
dc.contributor.schoolDepartment of Microbiology and Immunologyen
dc.date.issued1995en
dc.description.abstractExpression of genes encoding the toxin-co-regulated pilus (TCP) varies between the two biotypes of Vibrio cholerae O1. Sequence analysis of the tcp locus from the classical and El Tor strains has revealed differences in the intergenic regions between tcpI and tcpP, and tcpH and tcpA, which may be involved in regulation. To investigate this possibility, transcription of tcpA, and the predicted upstream promoters for tcpI and tcpP, has been analysed in the classical and El Tor strains using promoter-cat (chloramphenicol acetyltransferase) fusions. Together with primer extension analyses, these studies indicate that the tcpA and tcpP promoters are toxR-dependent and suggest that TcpP may be involved in activation of both the tcpI and tcpP promoters. We conclude that differences in the level of tcpA expression in a classical and an El Tor strain are likely to be due to the effect of sequence variation on the ability of control factors to act on these regulatory regions.en
dc.description.statementofresponsibilitySonia Thomas, Susan G. Williams and Paul A. Manningen
dc.identifier.citationGene, 1995; 166(1):43-48en
dc.identifier.doi10.1016/0378-1119(95)00610-Xen
dc.identifier.issn0378-1119en
dc.identifier.urihttp://hdl.handle.net/2440/11568
dc.language.isoenen
dc.publisherElsevieren
dc.rights© 1995 Elsevier Science B.V. All rights reserved.en
dc.titleRegulation of tcpgenes in classical and El Tor strains of Vibrio cholerae O1en
dc.typeJournal articleen

Files

Collections

Microbiology and Immunology publications