Different responses of larval fatty acid profiles to cryopreservation in two commercially important bivalves

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dc.contributor.authorZhu, X.
dc.contributor.authorMiller-Ezzy, P.
dc.contributor.authorHall, T.
dc.contributor.authorTang, Y.
dc.contributor.authorQin, J.
dc.contributor.authorZhao, Y.
dc.contributor.authorLi, X.
dc.date.issued2024
dc.description.abstractLarval cryopreservation techniques have been established in Pacific oysters and Mediterranean mussels. Although initial cryopreservation protocols for both species differed slightly in Ficoll PM 70 (FIC) concentration, better post-thaw larval survivability was produced in mussels than oysters. Furthermore, the post-thaw survivability in the latter could be improved significantly by the addition of lipids and antioxidants to the cryoprotectant agent. These findings have generated a unique opportunity to investigate the cryo-functions of both endogenous and exogenous fatty acids (FAs) in bivalves as mammalian studies indicated that lipid compositions could contribute the interspecific difference in gamete and embryo cryopreservation. Our study showed that the higher survivability of post-thaw larvae in mussels (65% vs. 34% in oysters) could be attributed to their higher proportion of total polyunsaturated fatty acids (PUFAs), especially C22:6, and higher resistance of FA profiles to cryopreservation. In oysters, on the other hand, their FA profiles were very sensitive to cryopreservation, with saturated FAs and PUFAs being significantly increased (from 37 to 41%) and decreased (from 35% to ~32%), respectively. Although exogenous lipids could significantly improve the survivability of post-thaw oyster larvae from 34 to 51%, their supplementation did not alter the response pattern of endogenous FA profiles to cryopreservation.
dc.description.statementofresponsibilityXiaochen Zhu, Penny Miller-Ezzy, Tony Hall, YouhongTang, Jianguang Qin, Yingying Zhao, Xiaoxu Li
dc.identifier.citationScientific Reports, 2024; 14(1):24582-1-24582-9
dc.identifier.doi10.1038/s41598-024-76723-0
dc.identifier.issn2045-2322
dc.identifier.issn2045-2322
dc.identifier.orcidHall, T. [0000-0001-8157-9445]
dc.identifier.urihttps://hdl.handle.net/2440/143572
dc.language.isoen
dc.publisherNature Portfolio
dc.rights© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
dc.source.urihttps://doi.org/10.1038/s41598-024-76723-0
dc.subjectFatty acid profile response; Magallana gigas; Mytilus galloprovincialis; Larval cryopreservation; Polyunsaturated fatty acids
dc.subject.meshAnimals
dc.subject.meshFatty Acids
dc.subject.meshFatty Acids, Unsaturated
dc.subject.meshCryoprotective Agents
dc.subject.meshCryopreservation
dc.subject.meshLarva
dc.subject.meshBivalvia
dc.titleDifferent responses of larval fatty acid profiles to cryopreservation in two commercially important bivalves
dc.typeJournal article
pubs.publication-statusPublished

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