Regeneration of almond from immature seed cotyledons
Date
2001
Authors
Ainsley, P.
Hammerschlag, F.
Bertozzi, T.
Collins, G.
Sedgley, M.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants, 2001; 67(3):221-226
Statement of Responsibility
Phillip J. Ainsley, Freddi A. Hammerschlag, Terry Bertozzi, Graham G. Collins and Margaret Sedgley
Conference Name
Abstract
Adventitious shoots were regenerated from immature cotyledons of four almond cultivars (`Ne Plus Ultra', `Nonpareil', `Carmel' and `Parkinson'). Open-pollinated fruit were collected from orchard-grown trees 100–115 days after full bloom. The proximal ends of the cotyledons were excised and the embryonic axes discarded. The effects of different concentrations of thidiazuron (TDZ) and indole-3-butyric acid (IBA) and the presence or absence of light for the first 7 days of culture were determined. Shoot regeneration rates were highest for cotyledons cultured for 8 weeks on Murashige and Skoog (MS) basal medium containing TDZ (10.0 mgrM), followed by 4 weeks on medium without plant growth regulators. Regeneration levels were further improved if cotyledons were maintained in darkness for the first 7 days. IBA (0.5 mgrM) significantly reduced the development of adventitious shoots. The frequency of cotyledons that developed adventitious shoots under the optimum tested conditions for `Ne Plus Ultra', `Nonpareil', `Carmel', and `Parkinson' were 80.0%, 73.3%, 100.0% and 86.7%, respectively.
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Dissertation Note
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The original publication can be found at www.springerlink.com
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© 2001 Kluwer Academic Publishers