Regeneration of almond from immature seed cotyledons
| dc.contributor.author | Ainsley, P. | |
| dc.contributor.author | Hammerschlag, F. | |
| dc.contributor.author | Bertozzi, T. | |
| dc.contributor.author | Collins, G. | |
| dc.contributor.author | Sedgley, M. | |
| dc.date.issued | 2001 | |
| dc.description | The original publication can be found at www.springerlink.com | |
| dc.description.abstract | Adventitious shoots were regenerated from immature cotyledons of four almond cultivars (`Ne Plus Ultra', `Nonpareil', `Carmel' and `Parkinson'). Open-pollinated fruit were collected from orchard-grown trees 100–115 days after full bloom. The proximal ends of the cotyledons were excised and the embryonic axes discarded. The effects of different concentrations of thidiazuron (TDZ) and indole-3-butyric acid (IBA) and the presence or absence of light for the first 7 days of culture were determined. Shoot regeneration rates were highest for cotyledons cultured for 8 weeks on Murashige and Skoog (MS) basal medium containing TDZ (10.0 mgrM), followed by 4 weeks on medium without plant growth regulators. Regeneration levels were further improved if cotyledons were maintained in darkness for the first 7 days. IBA (0.5 mgrM) significantly reduced the development of adventitious shoots. The frequency of cotyledons that developed adventitious shoots under the optimum tested conditions for `Ne Plus Ultra', `Nonpareil', `Carmel', and `Parkinson' were 80.0%, 73.3%, 100.0% and 86.7%, respectively. | |
| dc.description.statementofresponsibility | Phillip J. Ainsley, Freddi A. Hammerschlag, Terry Bertozzi, Graham G. Collins and Margaret Sedgley | |
| dc.identifier.citation | Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants, 2001; 67(3):221-226 | |
| dc.identifier.doi | 10.1023/A:1012700714085 | |
| dc.identifier.issn | 0167-6857 | |
| dc.identifier.orcid | Bertozzi, T. [0000-0001-6665-3395] | |
| dc.identifier.uri | http://hdl.handle.net/2440/13093 | |
| dc.language.iso | en | |
| dc.publisher | Kluwer Academic Publ | |
| dc.rights | © 2001 Kluwer Academic Publishers | |
| dc.source.uri | https://doi.org/10.1023/a:1012700714085 | |
| dc.subject | adventitious shoots | |
| dc.subject | indole-3-butyric acid | |
| dc.subject | organogenesis | |
| dc.subject | Prunus dulcis Mill | |
| dc.subject | thidiazuron | |
| dc.title | Regeneration of almond from immature seed cotyledons | |
| dc.type | Journal article | |
| pubs.publication-status | Published |