Ribosomal RNA genes and the B chromosome of Brachycome dichromosomatica
Date
1995
Authors
Donald, T.
Leach, C.
Clough, A.
Timmis, J.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Heredity, 1995; 74(5):556-561
Statement of Responsibility
Tamzin M Donald, Carolyn R Leach, Angela Clough and Jeremy N Timmis
Conference Name
Abstract
Fluorescence in situ hybridization (FISH) with biotinylated rDNA revealed the presence of an rRNA gene cluster on both the A and B chromosomes of Brachycome dichromosomatica, an Australian native ephemeral plant of the arid regions of south-eastern Australia. This species contains only two pairs of A chromosomes and up to three B chromosomes. The regular attachment of the B chromosome to a nucleolus suggests that these ribosomal RNA genes are transcribed. Southern hybridization of DNA from 0B and +B plants digested with a variety of restriction enzymes indicates that the rRNA genes on the A and B chromosomes are the same in sequence and methylation status.