Ribosomal RNA genes and the B chromosome of Brachycome dichromosomatica

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dc.contributor.authorDonald, T.
dc.contributor.authorLeach, C.
dc.contributor.authorClough, A.
dc.contributor.authorTimmis, J.
dc.date.issued1995
dc.description.abstractFluorescence in situ hybridization (FISH) with biotinylated rDNA revealed the presence of an rRNA gene cluster on both the A and B chromosomes of Brachycome dichromosomatica, an Australian native ephemeral plant of the arid regions of south-eastern Australia. This species contains only two pairs of A chromosomes and up to three B chromosomes. The regular attachment of the B chromosome to a nucleolus suggests that these ribosomal RNA genes are transcribed. Southern hybridization of DNA from 0B and +B plants digested with a variety of restriction enzymes indicates that the rRNA genes on the A and B chromosomes are the same in sequence and methylation status.
dc.description.statementofresponsibilityTamzin M Donald, Carolyn R Leach, Angela Clough and Jeremy N Timmis
dc.identifier.citationHeredity, 1995; 74(5):556-561
dc.identifier.doi10.1038/hdy.1995.77
dc.identifier.issn0018-067X
dc.identifier.issn1365-2540
dc.identifier.urihttp://hdl.handle.net/2440/11457
dc.language.isoen
dc.publisherOliver & Boyd
dc.source.urihttps://doi.org/10.1038/hdy.1995.77
dc.subjectChromosomes
dc.subjectPlants
dc.subjectRNA, Plant
dc.subjectRNA, Ribosomal
dc.subjectBlotting, Southern
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectMethylation
dc.titleRibosomal RNA genes and the B chromosome of Brachycome dichromosomatica
dc.typeJournal article
pubs.publication-statusPublished

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