Characterization of parainfluenza virus 5 from diarrheic piglet highlights its zoonotic potential

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dc.contributor.authorIbrahim, Y.M.
dc.contributor.authorZhang, W.
dc.contributor.authorWerid, G.M.
dc.contributor.authorZhang, H.
dc.contributor.authorPan, Y.
dc.contributor.authorZhang, L.
dc.contributor.authorXu, Y.
dc.contributor.authorLi, C.
dc.contributor.authorChen, H.
dc.contributor.authorWang, Y.
dc.date.issued2022
dc.description.abstractParainfluenza virus 5 (PIV5), a member of paramyxoviruses, causes respiratory and neurological infection in several animal species. Whereas information on PIV5 infection in digestive system is very scarce. Here, we successfully isolated one PIV5 strain from diarrheic piglets. After four times plaque purification and ultracentrifugation, the paramyxovirus-like particles were observed by electron microscopy. The genome-wide phylogenetic analysis showed that the isolated strain was closely related to the PIV5 strain from a lesser panda and pigs in China. Therefore, we characterized this isolated PIV5 and found that this virus could haemagglutinate red blood cells from both guinea pigs and chickens. Further, we observed that this PIV5 could infect cell lines from various host species including pig, human, monkey, bovine, dog, cat, rabbit, hamster and mouse, which was confirmedwith the immunofluorescent assay. To evaluate the distribution of PIV5 in the field, we developed an indirect ELISA (iELISA) for the first time to detect the specific antibodies based on recombinant nucleocapsid protein. A total of 530 porcine serum samples were tested and the PIV5-positive rate was 75.7%. To our knowledge, this is the first report describing the full characterization of PIV5 strain isolated from a diarrheic piglet. The ability of this PIV5 strain to infect a wide range of mammalian cell types indicates that PIV5 can transmit across different species, providing a remarkable insight into potential zoonosis. The virus strain and iELISA developed in this study can be used to investigate the pathogenesis, epidemiology, and zoonotic potential of PIV5.
dc.description.statementofresponsibilityYassein M. Ibrahim, Wenli Zhang, Gebremeskel Mamu Werid, He Zhang, Yu Pan, Lin Zhang, Yunfei Xu, Changwen Li, Hongyan Chen, Yue Wang
dc.identifier.citationTransboundary and Emerging Diseases, 2022; 69(5):e1510-e1525
dc.identifier.doi10.1111/tbed.14482
dc.identifier.issn1865-1674
dc.identifier.issn1865-1682
dc.identifier.orcidWerid, G.M. [0000-0002-3189-1470]
dc.identifier.urihttps://hdl.handle.net/2440/145973
dc.language.isoen
dc.publisherHindawi Limited
dc.rights© 2022 Wiley-VCH GmbH
dc.source.urihttps://doi.org/10.1111/tbed.14482
dc.subjectantibodies; characterization; cross-species transmission; iELISA; parainfluenza virus 5
dc.subject.meshCell Line
dc.subject.meshAnimals
dc.subject.meshChickens
dc.subject.meshMammals
dc.subject.meshCattle
dc.subject.meshSwine
dc.subject.meshDogs
dc.subject.meshRabbits
dc.subject.meshHumans
dc.subject.meshGuinea Pigs
dc.subject.meshMice
dc.subject.meshNucleocapsid Proteins
dc.subject.meshPhylogeny
dc.subject.meshParainfluenza Virus 5
dc.titleCharacterization of parainfluenza virus 5 from diarrheic piglet highlights its zoonotic potential
dc.typeJournal article
pubs.publication-statusPublished

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