The dengue virus M protein localises to the endoplasmic reticulum and forms oligomers
Date
2012
Authors
Wong, S.
Haqshenas, G.
Gowans, E.
MacKenzie, J.
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Journal article
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FEBS Letters, 2012; 586(7):1032-1037
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Sook-San Wonga, Gholamreza Haqshenas, Eric J. Gowans, Jason Mackenzie
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Abstract
The dengue virus membrane (M) protein is a key component of the mature virion. Here, we characterised the cellular behaviour of M using a recombinant protein construct to understand its inherent properties. Using confocal microscopy, we showed that M and its intracellular precursor, prM, localised to the endoplasmic reticulum. M protein was also detected on the cell surface and secreted, suggesting that M can enter the secretory pathway. In addition, cross-linking studies showed that M can form dimers and tetramers. These findings suggest that M behaves as a secretory protein analogous to the major envelope protein E.
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Crown Copyright © 2012 Published by Elsevier B.V. on behalf of Federation of European Biochemical society. All rights reserved.