Continuous production of oxytetracycline in fluidized bed bioreactor by immobilized cells of streptomyces varsoviensis MTCC-1537: effect of dilution and glucose loading rates
Date
2009
Authors
Jetty, A.
Potumarthi, R.
Rao, A.G.
Rao, B.S.
Ramakrishna, S.V.
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Journal article
Citation
Applied Biochemistry and Biotechnology, 2009; 159(2):579-588
Statement of Responsibility
Annapurna Jetty, Ravichandra Potumarthi, A. Gangagni Rao, B. Sarva Rao, S. V. Ramakrishna
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Abstract
Oxytetracycline (OT) production using glutaraldehyde cross-linked calcium alginate immobilized cells of Streptomyces varsoviensis in continuous fluidized bed reactor (FBR) was investigated. Initially, batch experiments were carried in stirred tank reactor (STR) and FBR using calcium alginate immobilized cells. Higher OT production of 0.45 gm/L was achieved by FBR when compared with 0.33 g/L of OT in STR. All subsequent studies were carried out in continuous mode of operation in FBR. During 21 days of operation, effect of glucose concentration and different dilution rates were studied. A maximum of 0.75 g/L OT was achieved in the medium having 10 g/L of glucose concentration. The highest OT concentration of 0.92 g/L and the highest yield of OT with respect to biomass at 0.1713 g/g were obtained at the dilution rate of 0.25 day-1.
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© Humana Press 2009