Simple approach to one-laser, broadband coherent anti-Stokes Raman scattering microscopy
Date
2004
Authors
Kee, T.
Cicerone, M.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Optics Letters, 2004; 29(23):2701-2703
Statement of Responsibility
Tak W. Kee and Marcus T. Cicerone
Conference Name
Abstract
Coherent anti-Stokes Raman scattering (CARS) microscopy is emerging as a powerful method for imaging materials and biological systems, partly because of its noninvasiveness and selective chemical sensitivity. However, its full potential for species-selective imaging is limited by a restricted spectral bandwidth. Recent increases in bandwidth are promising but still are not sufficient for the level of robust component discrimination that would be needed in a chemically complex milieu found, for example, in intracellular and extracellular environments. We demonstrate a truly broadband CARS imaging instrument that we use to acquire hyperspectral images with vibrational spectra over a bandwidth of 2500 cm^-1 with a resolution of 13 cm^-1.