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Simple approach to one-laser, broadband coherent anti-Stokes Raman scattering microscopy

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dc.contributor.authorKee, T.
dc.contributor.authorCicerone, M.
dc.date.issued2004
dc.description.abstractCoherent anti-Stokes Raman scattering (CARS) microscopy is emerging as a powerful method for imaging materials and biological systems, partly because of its noninvasiveness and selective chemical sensitivity. However, its full potential for species-selective imaging is limited by a restricted spectral bandwidth. Recent increases in bandwidth are promising but still are not sufficient for the level of robust component discrimination that would be needed in a chemically complex milieu found, for example, in intracellular and extracellular environments. We demonstrate a truly broadband CARS imaging instrument that we use to acquire hyperspectral images with vibrational spectra over a bandwidth of 2500 cm^-1 with a resolution of 13 cm^-1.
dc.description.statementofresponsibilityTak W. Kee and Marcus T. Cicerone
dc.identifier.citationOptics Letters, 2004; 29(23):2701-2703
dc.identifier.doi10.1364/OL.29.002701
dc.identifier.issn0146-9592
dc.identifier.issn1539-4794
dc.identifier.orcidKee, T. [0000-0002-4907-4663]
dc.identifier.urihttp://hdl.handle.net/2440/31822
dc.language.isoen
dc.publisherOptical Soc Amer
dc.source.urihttp://www.opticsinfobase.org/abstract.cfm?URI=ol-29-23-2701
dc.subjectMicroscopy, Interference
dc.subjectImage Enhancement
dc.subjectTomography, Optical Coherence
dc.subjectSpectrum Analysis, Raman
dc.subjectSensitivity and Specificity
dc.subjectFeasibility Studies
dc.subjectReproducibility of Results
dc.subjectEquipment Design
dc.subjectEquipment Failure Analysis
dc.subjectLasers
dc.titleSimple approach to one-laser, broadband coherent anti-Stokes Raman scattering microscopy
dc.typeJournal article
pubs.publication-statusPublished

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