The sodium transporter encoded by the HKT1;2 gene modulates sodium/potassium homeostasis in tomato shoots under salinity
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Date
2017
Authors
Jaime-Pérez, N.
Pineda, B.
García-Sogo, B.
Atares, A.
Athman, A.
Byrt, C.
Olías, R.
Asins, M.
Gilliham, M.
Moreno, V.
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Plant, Cell and Environment, 2017; 40(5):658-671
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Noelia Jaime-Pérez, Benito Pineda, Begoña García-Sogo, Alejandro Atares, Asmini Athman, Caitlin S. Byrt, Raquel Olías, Maria José Asins, Matthew Gilliham, Vicente Moreno, Andrés Belver
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Abstract
Excessive soil salinity diminishes crop yield and quality. In a previous study in tomato, we identified two closely linked genes encoding HKT1-like transporters, HKT1;1 and HKT1;2, as candidate genes for a major quantitative trait locus (kc7.1) related to shoot Na+ /K+ homeostasis - a major salt tolerance trait - using two populations of recombinant inbred lines (RILs). Here, we determine the effectiveness of these genes in conferring improved salt tolerance by using two near-isogenic lines (NILs) that were homozygous for either the Solanum lycopersicum allele (NIL17) or for the Solanum cheesmaniae allele (NIL14) at both HKT1 loci; transgenic lines derived from these NILs in which each HKT1;1 and HKT1;2 had been silenced by stable transformation were also used. Silencing of ScHKT1;2 and SlHKT1;2 altered the leaf Na+ /K+ ratio and caused hypersensitivity to salinity in plants cultivated under transpiring conditions, whereas silencing SlHKT1;1/ScHKT1;1 had a lesser effect. These results indicate that HKT1;2 has the more significant role in Na+ homeostasis and salinity tolerance in tomato.
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© 2016 John Wiley & Sons Ltd