The sodium transporter encoded by the HKT1;2 gene modulates sodium/potassium homeostasis in tomato shoots under salinity

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dc.contributor.authorJaime-Pérez, N.
dc.contributor.authorPineda, B.
dc.contributor.authorGarcía-Sogo, B.
dc.contributor.authorAtares, A.
dc.contributor.authorAthman, A.
dc.contributor.authorByrt, C.
dc.contributor.authorOlías, R.
dc.contributor.authorAsins, M.
dc.contributor.authorGilliham, M.
dc.contributor.authorMoreno, V.
dc.contributor.authorBelver, A.
dc.date.issued2017
dc.description.abstractExcessive soil salinity diminishes crop yield and quality. In a previous study in tomato, we identified two closely linked genes encoding HKT1-like transporters, HKT1;1 and HKT1;2, as candidate genes for a major quantitative trait locus (kc7.1) related to shoot Na+ /K+ homeostasis - a major salt tolerance trait - using two populations of recombinant inbred lines (RILs). Here, we determine the effectiveness of these genes in conferring improved salt tolerance by using two near-isogenic lines (NILs) that were homozygous for either the Solanum lycopersicum allele (NIL17) or for the Solanum cheesmaniae allele (NIL14) at both HKT1 loci; transgenic lines derived from these NILs in which each HKT1;1 and HKT1;2 had been silenced by stable transformation were also used. Silencing of ScHKT1;2 and SlHKT1;2 altered the leaf Na+ /K+ ratio and caused hypersensitivity to salinity in plants cultivated under transpiring conditions, whereas silencing SlHKT1;1/ScHKT1;1 had a lesser effect. These results indicate that HKT1;2 has the more significant role in Na+ homeostasis and salinity tolerance in tomato.
dc.description.statementofresponsibilityNoelia Jaime-Pérez, Benito Pineda, Begoña García-Sogo, Alejandro Atares, Asmini Athman, Caitlin S. Byrt, Raquel Olías, Maria José Asins, Matthew Gilliham, Vicente Moreno, Andrés Belver
dc.identifier.citationPlant, Cell and Environment, 2017; 40(5):658-671
dc.identifier.doi10.1111/pce.12883
dc.identifier.issn0140-7791
dc.identifier.issn1365-3040
dc.identifier.orcidByrt, C. [0000-0001-8549-2873]
dc.identifier.orcidGilliham, M. [0000-0003-0666-3078]
dc.identifier.urihttp://hdl.handle.net/2440/106101
dc.language.isoen
dc.publisherBlackwell Publishing
dc.relation.granthttp://purl.org/au-research/grants/arc/CE140100008
dc.relation.granthttp://purl.org/au-research/grants/arc/FT130100709
dc.rights© 2016 John Wiley & Sons Ltd
dc.source.urihttps://doi.org/10.1111/pce.12883
dc.subjectHKT1;2; post-transcriptional gene silencing; Solanum lycopersicum and Solanum cheesmaniae
dc.titleThe sodium transporter encoded by the HKT1;2 gene modulates sodium/potassium homeostasis in tomato shoots under salinity
dc.typeJournal article
pubs.publication-statusPublished

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