Rapid room-temperature synthesis of biocompatible metal–organic framework for enzyme immobilization with improved stability and on-demand release
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(Published version)
Date
2024
Authors
Weng, Y.
Yan, P.
Sun, B.
Wan, A.
You, J.
Xu, X.
Lu, Z.
Stewart, G.A.
Chen, X.
Song, H.
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Journal article
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Chemical Engineering Journal, 2024; 497(1):154471-1-154471-10
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Yilun Weng, Penghui Yan, Baode Sun, Andria Wan, Jiakang You, Xin Xu, Zeyu Lu, Glen A. Stewart, Xiaojing Chen, Hao Song, Chun-Xia Zhao
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Abstract
Enzyme immobilization within metal–organic frameworks (MOFs) addresses the inherent fragility of enzymes, playing a crucial role across diverse industries by improving efficiency and lowering economic costs. While the application of MOFs in the food and pharmaceutical industries is constrained by toxicity concerns, MIL-88A(Fe) emerges as an ideal candidate due to its non-toxicity and biocompatibility. However, the release of encapsulated enzymes is significantly hampered, reducing their bioactivity. Herein, we present a safe and simple platform for creating enzyme@MIL-88A, which provides enzyme stabilization and controlled release. The thermal stabilization of a spectrum of enzymes (phytase, xylanase, amylase, mannanase, and glucanase) is achieved, elevating their endurance threshold to 95 ◦C. Furthermore, the controlled on-demand release of the encapsulated enzymes at target sites is accomplished by adjusting defects in enzyme@MIL-88A composites via an acid modulation approach, while preserving enzyme activity. This approach has improved the amount of enzyme released from 10 % to 99.7 %. To the best of our knowledge, this is the first time enzyme@MIL-88A has been synthesized rapidly under mild conditions for enzyme stabilization and controlled release. Our method offers a universal platform for stabilizing vulnerable biomaterials and the controlled delivery of biological macromolecules.
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© 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).