Dissecting the causal polymorphism of the Lr67res multipathogen resistance gene
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(Published version)
Date
2024
Authors
Milne, R.J.
Dibley, K.E.
Bose, J.
Riaz, A.
Zhang, J.
Schnippenkoetter, W.
Ashton, A.R.
Ryan, P.R.
Tyerman, S.D.
Lagudah, E.S.
Editors
Wang, W.-M.
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Journal article
Citation
Journal of Experimental Botany, 2024; 75(13):3877-3890
Statement of Responsibility
Ricky J. Milne, Katherine E. Dibley, Jayakumar Bose, Adnan Riaz, Jianping Zhang, Wendelin Schnippenkoetter, Anthony R. Ashton, Peter R. Ryan, Stephen D. Tyerman, and Evans S. Lagudah
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Abstract
Partial resistance to multiple biotrophic fungal pathogens in wheat (Triticum aestivum L.) is conferred by a variant of the Lr67 gene, which encodes a hexose-proton symporter. Two mutations (G144R, V387L) differentiate the resistant and susceptible protein variants (Lr67res and Lr67sus). Lr67res lacks sugar transport capability and was associated with anion transporter-like properties when expressed in Xenopus laevis oocytes. Here, we extended this functional characterization to include yeast and in planta studies. The Lr67res allele, but not Lr67sus, induced sensitivity to ions in yeast (including NaCl, LiCl, KI), which is consistent with our previous observations that Lr67res expression in oocytes induces novel ion fluxes. We demonstrate that another naturally occurring single amino acid variant in wheat, containing only the Lr67G144R mutation, confers rust resistance. Transgenic barley plants expressing the orthologous HvSTP13 gene carrying the G144R and V387L mutations were also more resistant to Puccinia hordei infection. NaCl treatment of pot-grown adult wheat plants with the Lr67res allele induced leaf tip necrosis and partial leaf rust resistance. An Lr67res-like function can be introduced into orthologous plant hexose transporters via single amino acid mutation, highlighting the strong possibility of generating disease resistance in other crops, especially with gene editing.
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Advance Access Publication 15 April 2024
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© The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.