Challenges of 'sticky' co-immunoprecipitation: Polyalanine tract protein-protein interactions
Date
2013
Authors
Mattiske, T.
Tan, M.
Gecz, J.
Shoubridge, C.
Editors
Hatters, D.
Hannan, A.
Hannan, A.
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Book chapter
Citation
Tandem repeats in genes, proteins, and disease : methods and protocols, 2013 / Hatters, D., Hannan, A. (ed./s), vol.1017, Ch.9, pp.121-133
Statement of Responsibility
T. R. Mattiske, May H. Tan, Jozef Gécz, and Cheryl Shoubridge
Conference Name
Abstract
Co-immunoprecipitation (Co-IP) (followed by immunoblotting) is a technique widely used to characterize specific protein–protein interactions. Investigating interactions of proteins containing “sticky” polyalanine (PolyA) tracts encounters difficulties using conventional Co-IP procedures. Here, we present strategies to specifically capture proteins containing these difficult PolyA tracts, enabling subsequent robust detection of interacting proteins by Co-IP.
School/Discipline
Dissertation Note
Provenance
Description
ISSN 1064-3745 (print) ISSN 1940-6029 (electronic)
Access Status
Rights
© Springer Science+Business Media New York 2013