Challenges of 'sticky' co-immunoprecipitation: Polyalanine tract protein-protein interactions

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dc.contributor.authorMattiske, T.
dc.contributor.authorTan, M.
dc.contributor.authorGecz, J.
dc.contributor.authorShoubridge, C.
dc.contributor.editorHatters, D.
dc.contributor.editorHannan, A.
dc.date.issued2013
dc.descriptionISSN 1064-3745 (print) ISSN 1940-6029 (electronic)
dc.description.abstractCo-immunoprecipitation (Co-IP) (followed by immunoblotting) is a technique widely used to characterize specific protein–protein interactions. Investigating interactions of proteins containing “sticky” polyalanine (PolyA) tracts encounters difficulties using conventional Co-IP procedures. Here, we present strategies to specifically capture proteins containing these difficult PolyA tracts, enabling subsequent robust detection of interacting proteins by Co-IP.
dc.description.statementofresponsibilityT. R. Mattiske, May H. Tan, Jozef Gécz, and Cheryl Shoubridge
dc.identifier.citationTandem repeats in genes, proteins, and disease : methods and protocols, 2013 / Hatters, D., Hannan, A. (ed./s), vol.1017, Ch.9, pp.121-133
dc.identifier.doi10.1007/978-1-62703-438-8_9
dc.identifier.isbn9781627034371
dc.identifier.orcidMattiske, T. [0000-0002-9581-5370]
dc.identifier.orcidGecz, J. [0000-0002-7884-6861]
dc.identifier.orcidShoubridge, C. [0000-0002-0157-3084]
dc.identifier.urihttp://hdl.handle.net/2440/81224
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofseriesMethods in Molecular Biology ; 1017
dc.rights© Springer Science+Business Media New York 2013
dc.subjectPolyalanine tract; ARX; Co-immunoprecipitation; Protein–protein interaction
dc.titleChallenges of 'sticky' co-immunoprecipitation: Polyalanine tract protein-protein interactions
dc.typeBook chapter
pubs.publication-statusPublished

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