Spatially offset optical coherence tomography: Leveraging multiple scattering for high-contrast imaging at depth in turbid media

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dc.contributor.authorUntracht, G.R.
dc.contributor.authorChen, M.
dc.contributor.authorWijesinghe, P.
dc.contributor.authorMas, J.
dc.contributor.authorYura, H.T.
dc.contributor.authorMarti, D.
dc.contributor.authorAndersen, P.E.
dc.contributor.authorDholakia, K.
dc.date.issued2023
dc.description.abstractThe penetration depth of optical coherence tomography (OCT) reaches well beyond conventional microscopy; however, signal reduction with depth leads to rapid degradation of the signal below the noise level. The pursuit of imaging at depth has been largely approached by extinguishing multiple scattering. However, in OCT, multiple scattering substantially contributes to image formation at depth. Here, we investigate the role of multiple scattering in OCT image contrast and postulate that, in OCT, multiple scattering can enhance image contrast at depth. We introduce an original geometry that completely decouples the incident and collection fields by introducing a spatial offset between them, leading to preferential collection of multiply scattered light. A wave optics-based theoretical framework supports our experimentally demonstrated improvement in contrast. The effective signal attenuation can be reduced by more than 24 decibels. Notably, a ninefold enhancement in image contrast at depth is observed in scattering biological samples. This geometry enables a powerful capacity to dynamically tune for contrast at depth.
dc.description.statementofresponsibilityGavrielle R. Untracht, Mingzhou Chen, Philip Wijesinghe, Josep Mas, Harold T. Yura, Dominik Marti, Peter E. Andersen, Kishan Dholakia
dc.identifier.citationScience Advances, 2023; 9(27):eadh5435-eadh5435
dc.identifier.doi10.1126/sciadv.adh5435
dc.identifier.issn2375-2548
dc.identifier.issn2375-2548
dc.identifier.orcidDholakia, K. [0000-0001-6534-9009]
dc.identifier.urihttps://hdl.handle.net/2440/139420
dc.language.isoen
dc.publisherAmerican Association for the Advancement of Science
dc.relation.granthttp://purl.org/au-research/grants/arc/FL210100099
dc.rights© 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).
dc.source.urihttps://doi.org/10.1126/sciadv.adh5435
dc.subjectMicroscopy
dc.subjectTomography, Optical Coherence
dc.subjectScattering, Radiation
dc.subjectOptics and Photonics
dc.subject.meshMicroscopy
dc.subject.meshTomography, Optical Coherence
dc.subject.meshScattering, Radiation
dc.subject.meshOptics and Photonics
dc.titleSpatially offset optical coherence tomography: Leveraging multiple scattering for high-contrast imaging at depth in turbid media
dc.typeJournal article
pubs.publication-statusPublished

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