Both Notch1 and its ligands in B cells promote antibody production
Date
2017
Authors
Zhu, G.
Wang, X.
Xiao, H.
Liu, X.
Fang, Y.
Zhai, B.
Xu, R.
Han, G.
Chen, G.
Hou, C.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Molecular Immunology, 2017; 91:17-23
Statement of Responsibility
Gaizhi Zhu, Xiaoqian Wang, He Xiao, Xiaoling Liu, Ying Fang, Bing Zhai, Ruonan Xu, Gencheng Han, Guojiang Chen, Chunmei Hou, Beifen Shen, Yan Li, Ning Ma, Haitao Wu, Guangchao Liu, Renxi Wang
Conference Name
Abstract
Notch1 signaling regulates B and T lymphocyte development and also in vitro promotes antibody secretion upon B cell activation. However, it is still unclear about the role of Notch1 in antibody production upon in vitro and in vivo mixture lymphocytes activation. We first showed that Notch1 expressed in LPS-activated CD19hi B cells and CD19cre mediated Notch1 knock-down in LPS-activated B cells. Furthermore, we demonstrated that Notch1 knock-down in B cells reduced antibody production in LPS-stimulated B cells but did not affect antibody production in LPS-stimulated splenocytes and in experimental allergic encephalomyelitis (EAE) mice. Importantly, Notch1 ligands Dll1 and Jag1 expressed in B cells and pre-coated Notch1 protein promotes Notch1-knocked down B cells to produce antibody in LPS-stimulated B cells suggesting that Notch1 in other cells may promote antibody production by binding its ligands Dll1 and Jag1 in B cells. Together, our results suggest that both Notch1 and its ligands in B cells play an important role in antibody production.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
© 2017 Elsevier Ltd. All rights reserved