Spatial and temporal control of CRISPR-Cas9-mediated gene editing delivered via a light-triggered liposome system
Date
2020
Authors
Aksoy, Y.A.
Yang, B.
Chen, W.
Hung, T.
Kuchel, R.P.
Zammit, N.W.
Grey, S.T.
Goldys, E.M.
Deng, W.
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Journal article
Citation
ACS applied materials & interfaces, 2020; 12(47):52433-52444
Statement of Responsibility
Yagiz Alp Aksoy, Biyao Yang, Wenjie Chen, Tzongtyng Hung, Rhiannon P. Kuchel, Nathan W. Zammit, Shane T. Grey, Ewa M. Goldys, and Wei Deng
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Abstract
The CRISPR-Cas9 and related systems offer a unique genome-editing tool allowing facile and efficient introduction of heritable and locus-specific sequence modifications in the genome. Despite its molecular precision, temporal and spatial control of gene editing with the CRISPR-Cas9 system is very limited. We developed a light-sensitive liposome delivery system that offers a high degree of spatial and temporal control of gene editing with the CRISPR-Cas9 system. We demonstrated its efficient protein release by respectively assessing the targeted knockout of the eGFP gene in human HEK293/GFP cells and the TNFAIP3 gene in TNFα-induced HEK293 cells. We further validated our results at a single-cell resolution using an in vivo eGFP reporter system in zebrafish (77% knockout). These findings indicate that light-triggered liposomes may have new options for precise control of CRISPR-Cas9 release and editing.
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© 2020 American Chemical Society