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Spatial and temporal control of CRISPR-Cas9-mediated gene editing delivered via a light-triggered liposome system

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dc.contributor.authorAksoy, Y.A.
dc.contributor.authorYang, B.
dc.contributor.authorChen, W.
dc.contributor.authorHung, T.
dc.contributor.authorKuchel, R.P.
dc.contributor.authorZammit, N.W.
dc.contributor.authorGrey, S.T.
dc.contributor.authorGoldys, E.M.
dc.contributor.authorDeng, W.
dc.date.issued2020
dc.description.abstractThe CRISPR-Cas9 and related systems offer a unique genome-editing tool allowing facile and efficient introduction of heritable and locus-specific sequence modifications in the genome. Despite its molecular precision, temporal and spatial control of gene editing with the CRISPR-Cas9 system is very limited. We developed a light-sensitive liposome delivery system that offers a high degree of spatial and temporal control of gene editing with the CRISPR-Cas9 system. We demonstrated its efficient protein release by respectively assessing the targeted knockout of the eGFP gene in human HEK293/GFP cells and the TNFAIP3 gene in TNFα-induced HEK293 cells. We further validated our results at a single-cell resolution using an in vivo eGFP reporter system in zebrafish (77% knockout). These findings indicate that light-triggered liposomes may have new options for precise control of CRISPR-Cas9 release and editing.
dc.description.statementofresponsibilityYagiz Alp Aksoy, Biyao Yang, Wenjie Chen, Tzongtyng Hung, Rhiannon P. Kuchel, Nathan W. Zammit, Shane T. Grey, Ewa M. Goldys, and Wei Deng
dc.identifier.citationACS applied materials & interfaces, 2020; 12(47):52433-52444
dc.identifier.doi10.1021/acsami.0c16380
dc.identifier.issn1944-8244
dc.identifier.issn1944-8252
dc.identifier.orcidGrey, S.T. [0000-0003-2160-1625]
dc.identifier.urihttps://hdl.handle.net/2440/133290
dc.language.isoen
dc.publisherAmerican Chemical Society (ACS)
dc.relation.granthttp://purl.org/au-research/grants/arc/CE140100003
dc.relation.granthttp://purl.org/au-research/grants/arc/DP170101863
dc.rights© 2020 American Chemical Society
dc.source.urihttps://doi.org/10.1021/acsami.0c16380
dc.subjectCRISPR-Cas9; liposomes; light triggering; temporal control; protein release
dc.subject.meshEmbryo, Nonmammalian
dc.subject.meshAnimals
dc.subject.meshZebrafish
dc.subject.meshHumans
dc.subject.meshSinglet Oxygen
dc.subject.meshTumor Necrosis Factor-alpha
dc.subject.meshGreen Fluorescent Proteins
dc.subject.meshLiposomes
dc.subject.meshGene Expression
dc.subject.meshGenes, Reporter
dc.subject.meshLight
dc.subject.meshHEK293 Cells
dc.subject.meshCRISPR-Cas Systems
dc.subject.meshTumor Necrosis Factor alpha-Induced Protein 3
dc.subject.meshGene Editing
dc.titleSpatial and temporal control of CRISPR-Cas9-mediated gene editing delivered via a light-triggered liposome system
dc.typeJournal article
pubs.publication-statusPublished

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