Investigating causality with fecal microbiota transplantation in rodents: applications, recommendations and pitfalls.
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(Published version)
Date
2021
Authors
Gheorghe, C.E.
Ritz, N.L.
Martin, J.A.
Wardill, H.R.
Cryan, J.F.
Clarke, G.
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Journal article
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Gut Microbes, 2021; 13(1):e1941711-1-e1941711-51
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Cassandra E. Gheorghe, Nathaniel L. Ritz, Jason A. Martin, Hannah R. Wardill, John F. Cryan, Gerard Clarke
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Abstract
In recent years, studies investigating the role of the gut microbiota in health and diseases have increased enormously – making it essential to deepen and question the research methodology employed. Fecal microbiota transplantation (FMT) in rodent studies (either from human or animal donors) allows us to better understand the causal role of the intestinal microbiota across multiple fields. However, this technique lacks standardization and requires careful experimental design in order to obtain optimal results. By comparing several studies in which rodents are the final recipients of FMT, we summarize the common practices employed. In this review, we document the limitations of this method and highlight different parameters to be considered while designing FMT Studies. Standardizing this method is challenging, as it differs according to the research topic, but avoiding common pitfalls is feasible. Several methodological questions remain unanswered to this day and we offer a discussion on issues to be explored in future studies.
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© 2021 The Author(s). Published with license by Taylor & Francis Group, LLC. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.