Microfibril-associated glycoprotein-1 (MAGP-1) is specifically located on the beads of the beaded-filament structure for fibrillin-containing microfibrils as visualized by the rotary shadowing technique

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dc.contributor.authorHenderson, M.
dc.contributor.authorPolewski, R.
dc.contributor.authorFanning, J.
dc.contributor.authorGibson, M.
dc.date.issued1996
dc.description.abstractThis study used immunoelectron microscopic techniques to define the ultrastructural location of MAGP-1 on the fibrillin-containing microfibrils of the ocular zonule. A specific anti-MAGP-1 monoclonal antibody (MAb), 11B, was produced that did not crossreact with fibrillin-1 or other microfibrillar proteins. MAb 11B was shown by immunofluorescence to localize intensely to zonular tissue. Postembedding immunoelectron microscopy showed that MAGP-1 was associated with microfibrils throughout the zonule, with the exception of a narrow band of microfibrils at the junction with the lens capsule. With preembedding labeling, the anti-MAGP-1 MAb was found to localize in a crossbanding pattern, at intervals of about 50 nm, to microfibrils throughout the zonule and along bundles of microfibrils in surrounding vitreous tissue. Rotary shadowing of isolated microfibrils showed a "beads on a string" morphology with a periodicity of about 50 nm. With immunogold labeling, the anti-MAGP-1 antibody specifically localized on the beads in a symmetrical manner. Occasionally two gold partides were attached to the same bead, suggesting that multiple MAGP-1 molecules were present in the structure. The results indicate that MAGP-1 is intimately and regularly associated with the bead regions of fibrillin-containing microfibrils. The findings are consistent with a major structural role for MAGP-1 in microfibril biology.
dc.description.statementofresponsibilityM Henderson, R Polewski, J C Fanning and M A Gibson
dc.identifier.citationJournal of Histochemistry and Cytochemistry: imaging the spectrum of cell biology, 1996; 44(12):1389-1397
dc.identifier.doi10.1177/44.12.8985131
dc.identifier.issn0022-1554
dc.identifier.issn1551-5044
dc.identifier.urihttp://hdl.handle.net/2440/27325
dc.language.isoen
dc.publisherHistochemical Society
dc.rights©1996 by The Histochemical Society, Inc.
dc.source.urihttps://doi.org/10.1177/44.12.8985131
dc.subjectEye
dc.subjectAnimals
dc.subjectCattle
dc.subjectMicrofilament Proteins
dc.subjectContractile Proteins
dc.subjectAntibodies, Monoclonal
dc.subjectMicroscopy, Electron
dc.subjectFluorescent Antibody Technique
dc.subjectAntibody Specificity
dc.subjectFibrillins
dc.titleMicrofibril-associated glycoprotein-1 (MAGP-1) is specifically located on the beads of the beaded-filament structure for fibrillin-containing microfibrils as visualized by the rotary shadowing technique
dc.typeJournal article
pubs.publication-statusPublished

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