Comparison of the biocidal efficacy of sodium dichloroisocyanurate and calcium hydroxide as intra-canal medicaments over a seven-day contact time: an ex-vivo study.
Date
2020
Authors
Chan, W.
Chowdhury, N.R.
Sharma, G.
Zilm, P.
Rossi-Fedele, G.
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Journal of Endodontics, 2020; 46(9):1273-1278
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Wing Chan, Neelika Roy, Garima Sharma, Peter Zilm, and Giampiero Rossi-Fedele
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Abstract
Introduction: The use of medicaments has been recommended for the treatment of root canal infection. This study evaluated the antimicrobial effect of a well-known biocidal agent, sodium dichloroisocyanurate (NaDCC), by comparing it with calcium hydroxide [Ca(OH)₂] using an engineered 3-species biofilm root canal model. Methods: Thirty-eight human single-rooted teeth were decoronated and chemomechanically prepared before inoculation using Enterococcus faecalis, Actinomyces viscosus, and Streptococcus mutans using a flow cell model for 4 weeks for biofilm formation. The samples were randomly divided into 3 groups: NaDCC (n=12), Ca(OH)₂ (n=12), and positive control (no medicament, n=14). The medicaments were placed using a Lentulo spiral. After a contact time of 7 days, the roots were crushed and plated on selective media. Bacterial identities were confirmed with the use of selective media. Colony-forming units were calculated (CFU/ml). Descriptive statistics (mean and standard deviations) were calculated. Results: Seven-day dressing with NaDCC or Ca(OH)₂ presented with no growth. For the positive control, the mean colony-forming units were 2.97E4 ± 3.42E4 (CFU/ml). All previously inoculated strains were recovered in the control group. Conclusions: NaDCC and Ca(OH)₂ when used as intracanal medicaments were able to eradicate an engineered 3-species biofilm in single-rooted teeth in an ex vivo model. NaDCC deserves further investigation as an intracanal medicament. (J Endod 2020;46:1273–1278.)
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© 2020 American Association of Endodontists.