Epoxy-amine synthesised hydrogel scaffolds for soft-tissue engineering
Date
2010
Authors
Hamid, Z.A.A.
Blencowe, A.
Ozcelik, B.
Palmer, J.A.
Stevens, G.W.
Abberton, K.M.
Morrison, W.A.
Penington, A.J.
Qiao, G.G.
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Journal article
Citation
Biomaterials, 2010; 31(25):6454-6467
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Abstract
Highly porous and biodegradable hydrogels based on poly(ethylene glycol) (PEG) and cystamine (Cys) were fabricated using epoxy-amine chemistry and investigated as scaffolds for soft-tissue engineering. Whereas the application of fused-salt templates provided a comprehensive interconnecting pore morphology, the incorporation of a specially designed poly(epsilon-caprolactone) (PCL) cross-linker provided enhanced mechanical function without adversely effecting the scaffolds positive biological interactions. The addition of only 1.2 wt% of the PCL cross-linker was sufficient to provide improvements in the ultimate stress of 30-40%. In vitro studies not only confirmed the non-cytotoxic nature of the scaffolds, but also their degradation products, which were isolated and characterised by nuclear magnetic resonance (NMR) and matrix-assisted laser desorption/ionisation time-of-flight mass spectroscopy (MALDI ToF MS). In vivo trials were conducted over a period of 8 weeks through implantation of the scaffolds into the dorsal region of rats. At both 2 and 8 week time points the explants revealed complete infiltration by the surrounding tissue and the development of a vascular network to support the newly generated tissue, without an excessive foreign-body response.
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Data source: Supplementary data, https://doi.org/10.1016/j.biomaterials.2010.05.008
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Copyright 2010 Elsevier