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Epoxy-amine synthesised hydrogel scaffolds for soft-tissue engineering

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dc.contributor.authorHamid, Z.A.A.
dc.contributor.authorBlencowe, A.
dc.contributor.authorOzcelik, B.
dc.contributor.authorPalmer, J.A.
dc.contributor.authorStevens, G.W.
dc.contributor.authorAbberton, K.M.
dc.contributor.authorMorrison, W.A.
dc.contributor.authorPenington, A.J.
dc.contributor.authorQiao, G.G.
dc.date.issued2010
dc.descriptionData source: Supplementary data, https://doi.org/10.1016/j.biomaterials.2010.05.008
dc.description.abstractHighly porous and biodegradable hydrogels based on poly(ethylene glycol) (PEG) and cystamine (Cys) were fabricated using epoxy-amine chemistry and investigated as scaffolds for soft-tissue engineering. Whereas the application of fused-salt templates provided a comprehensive interconnecting pore morphology, the incorporation of a specially designed poly(epsilon-caprolactone) (PCL) cross-linker provided enhanced mechanical function without adversely effecting the scaffolds positive biological interactions. The addition of only 1.2 wt% of the PCL cross-linker was sufficient to provide improvements in the ultimate stress of 30-40%. In vitro studies not only confirmed the non-cytotoxic nature of the scaffolds, but also their degradation products, which were isolated and characterised by nuclear magnetic resonance (NMR) and matrix-assisted laser desorption/ionisation time-of-flight mass spectroscopy (MALDI ToF MS). In vivo trials were conducted over a period of 8 weeks through implantation of the scaffolds into the dorsal region of rats. At both 2 and 8 week time points the explants revealed complete infiltration by the surrounding tissue and the development of a vascular network to support the newly generated tissue, without an excessive foreign-body response.
dc.identifier.citationBiomaterials, 2010; 31(25):6454-6467
dc.identifier.doi10.1016/j.biomaterials.2010.05.008
dc.identifier.issn0142-9612
dc.identifier.issn1878-5905
dc.identifier.orcidBlencowe, A. [0000-0002-7630-4874]
dc.identifier.urihttps://hdl.handle.net/1959.8/153064
dc.language.isoen
dc.publisherELSEVIER SCI LTD
dc.relation.fundingARC LE0882576
dc.relation.granthttp://purl.org/au-research/grants/arc/LE0882576
dc.rightsCopyright 2010 Elsevier
dc.source.urihttps://doi.org/10.1016/j.biomaterials.2010.05.008
dc.subject3T3 Cells
dc.subjectAnimals
dc.subjectMice
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectPolyethylene Glycols
dc.subjectAmines
dc.subjectCystamine
dc.subjectEpoxy Compounds
dc.subjectBiocompatible Materials
dc.subjectHydrogels
dc.subjectTissue Engineering
dc.subjectMaterials Testing
dc.subjectAbsorbable Implants
dc.subjectCell Survival
dc.subjectPorosity
dc.subjectMale
dc.subjectTissue Scaffolds
dc.titleEpoxy-amine synthesised hydrogel scaffolds for soft-tissue engineering
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9915910472001831

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