Inhibition of α-glucosidase activity by curcumin loaded on ZnO@rGO nanocarrier for potential treatment of diabetes mellitus

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dc.contributor.authorLiu, L.
dc.contributor.authorWang, Z.
dc.contributor.authorYap, P.L.
dc.contributor.authorZhang, Q.
dc.contributor.authorNi, Y.
dc.contributor.authorLosic, D.
dc.date.issued2024
dc.description.abstractCurcumin (Cur) is an acidic polyphenol with some effects on α-glucosidase (α-Glu), but Cur has disadvantages such as being a weak target, lacking passing the blood-brain barrier and having low bioavailability. To enhance the curative effect of Cur, the hybrid composed of ZnO nanoparticles decorated on rGO was used to load Cur (ZnO@rGO-Cur). The use of the multispectral method and enzyme inhibition kinetics analysis certify the inhibitory effect and interaction mechanism of ZnO@rGO-Cur with α-Glu. The static quenching of α-Glu with both Cur and ZnO@rGO-Cur is primarily driven by hydrogen bond and van der Waals interactions. The conformation-changing ability by binding to the neighbouring phenolic hydroxyl group of Cur increased their ability to alter the secondary structure of α-Glu, resulting in the inhibition of enzyme activity. The inhibition constant (Ki, Cur  > Kis,ZnO@rGO-Cur ) showed that the inhibition effect of ZnO@rGO-Cur on α-Glu was larger than that of Cur. The CCK-8 experiments proved that ZnO@rGO nanocomposites have good biocompatibility. These results suggest that the therapeutic potential of ZnO@rGO-Cur composite is an emerging nanocarrier platform for drug delivery systems for the potential treatment of diabetes mellitus.
dc.description.statementofresponsibilityLinghong Liu, Zhu Wang, Pei Lay Yap, Qiulan Zhang, Yongnian Ni, Dusan Losic
dc.identifier.citationLuminescence, 2024; 39(1):e4668-1-e4668-12
dc.identifier.doi10.1002/bio.4668
dc.identifier.issn1522-7235
dc.identifier.issn1522-7243
dc.identifier.orcidYap, P.L. [0000-0001-7346-8139]
dc.identifier.orcidLosic, D. [0000-0002-1930-072X]
dc.identifier.urihttps://hdl.handle.net/2440/143561
dc.language.isoen
dc.publisherWiley
dc.relation.grant21665017
dc.rights© 2024 John Wiley & Sons Ltd.
dc.source.urihttp://dx.doi.org/10.1002/bio.4668
dc.subjectcurcumin; graphene; inhibition; molecule interaction; ZnO@rGO; α-glucosidase
dc.subject.meshalpha-Glucosidases
dc.subject.meshCurcumin
dc.subject.meshDiabetes Mellitus
dc.subject.meshDrug Delivery Systems
dc.subject.meshHumans
dc.subject.meshNanoparticles
dc.subject.meshZinc Oxide
dc.subject.meshGlycoside Hydrolase Inhibitors
dc.titleInhibition of α-glucosidase activity by curcumin loaded on ZnO@rGO nanocarrier for potential treatment of diabetes mellitus
dc.typeJournal article
pubs.publication-statusPublished

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