Vaccine protection of broilers against various doses of wild-type Salmonella Typhimurium and changes in gut microbiota

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dc.contributor.authorKhan, S.
dc.contributor.authorMcwhorter, A.R.
dc.contributor.authorWillson, N.-L.
dc.contributor.authorAndrews, D.M.
dc.contributor.authorUnderwood, G.J.
dc.contributor.authorMoore, R.J.
dc.contributor.authorVan, T.T.H.
dc.contributor.authorChousalkar, K.K.
dc.date.issued2025
dc.description.abstractThis study evaluated the impact of vaccine diluents (peptone or water) on the protective effects of Salmonella Typhimurium (S. Typhimurium) vaccine. Vaccinated broilers were challenged with different doses of wild-type S. Typhimurium through dust. At the time of cull, vaccine load was highest in caeca and lowest in spleen. Wild-type S. Typhimurium was detectable after 24 hrs only in the vaccinated birds challenged with 108 CFU and positive control. S. Typhimurium load was lower in the organs of the groups challenged with 104 and 106 compared to the 108 CFU group. The caecal microbiota alpha diversity of the vaccinated or vaccinated and challenged chickens differed from the positive and negative control groups. Beta diversity of the positive control clustered separately from all other treatment groups, showing that vaccine caused minimal changes in gut microbiota structure. The vaccinated and/or wild-type challenged chickens showed significantly higher abundance of Anaerostignum, Lachnoclostridium, Intestinimonas, Colidextribacter, Monoglobus, Acetanaerobacterium and Subdoligranulum. Outcomes from this study demonstrate that the vaccine effectively protected broiler chickens from S. Typhimurium infection and helped maintain a more stable gut microbiota structure, reducing the impact of S. Typhimurium on gut health. Vaccine diluent did not affect gut microbiota composition.
dc.description.statementofresponsibilitySamiullah Khan, Andrea R. McWhorter, Nicky-Lee Willson, Daniel M. Andrews, Gregory J. Underwood, Robert J. Moore, Thi Thu Hao Van, Kapil K. Chousalkar
dc.identifier.citationVeterinary Quarterly, 2025; 45(1):1-14
dc.identifier.doi10.1080/01652176.2024.2440428
dc.identifier.issn0165-2176
dc.identifier.issn1875-5941
dc.identifier.orcidKhan, S. [0000-0002-0292-2979]
dc.identifier.orcidMcwhorter, A.R. [0000-0002-5793-1629]
dc.identifier.orcidWillson, N.-L. [0000-0002-3851-8548]
dc.identifier.orcidChousalkar, K.K. [0000-0002-4134-3996]
dc.identifier.urihttps://hdl.handle.net/2440/143674
dc.language.isoen
dc.publisherTaylor & Francis
dc.relation.granthttp://purl.org/au-research/grants/arc/LP190100864
dc.rights© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.
dc.source.urihttps://doi.org/10.1080/01652176.2024.2440428
dc.subjectVaxsafe® ST; broiler chickens; poultry production; salmonellosis; gut microbiota; microbial diversity; dose of infection; vaccine diluent; Salmonella Typhimurium
dc.subject.meshCecum
dc.subject.meshAnimals
dc.subject.meshChickens
dc.subject.meshSalmonella typhimurium
dc.subject.meshSalmonella Infections, Animal
dc.subject.meshPoultry Diseases
dc.subject.meshSalmonella Vaccines
dc.subject.meshVaccination
dc.subject.meshGastrointestinal Microbiome
dc.titleVaccine protection of broilers against various doses of wild-type Salmonella Typhimurium and changes in gut microbiota
dc.typeJournal article
pubs.publication-statusPublished

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