Dust sprinkling as an effective method for infecting layer chickens with wild-type Salmonella Typhimurium and changes in host gut microbiota
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(Published version)
Date
2024
Authors
Khan, S.
McWhorter, A.R.
Andrews, D.M.
Underwood, G.J.
Moore, R.J.
Van, T.T.H.
Gast, R.K.
Chousalkar, K.K.
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Journal article
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Environmental Microbiology Reports, 2024; 16(3):e13265-1-e13265-13
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Samiullah Khan, Andrea R. McWhorter, Daniel M. Andrews, Gregory J. Underwood, Robert J. Moore, Thi Thu Hao Van, Richard K. Gast, Kapil K. Chousalkar
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Abstract
Role of dust in Salmonella transmission on chicken farms is not well characterised. Salmonella Typhimurium (ST) infection of commercial layer chickens was investigated using a novel sprinkling method of chicken dust spiked with ST and the uptake compared to a conventional oral infection. While both inoculation methods resulted in colonisation of the intestines, the Salmonella load in liver samples was significantly higher at 7 dpi after exposing chicks to sprinkled dust compared to the oral infection group. Infection of chickens using the sprinkling method at a range of doses showed a threshold for colonisation of the gut and organs as low as 1000 CFU/g of dust. Caecal content microbiota analysis post-challenge showed that the profiles of chickens infected by the sprinkling and oral routes were not significantly different; however, both challenges induced differences when compared to the uninfected negative controls. Overall, the study showed that dust sprinkling was an effective way to experimentally colonise chickens with Salmonella and alter the gut microbiota than oral gavage at levels as low as 1000 CFU/g dust. This infection model mimics the field scenario of Salmonella infection in poultry sheds. The model can be used for future challenge studies for effective Salmonella control.
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© 2024 The Authors. Environmental Microbiology Reports published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.