Hydrogel-based preparation of cell aggregates for biomedical applications
Date
2020
Authors
Zhang, J.
Yun, S.
Du, Y.
Zannettino, A.
Zhang, H.
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Journal article
Citation
Applied Materials Today, 2020; 20:100747-1-100747-19
Statement of Responsibility
Jiabin Zhang, Seonho Yun, Yuguang Du, Andrew Zannettino, Hu Zhang
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Abstract
Cell aggregates are widely used either as in-vitro models for drug screening, fundamental studies on disease progression and developmental biology, as well as in-vivo injectable cells for tissue regeneration. Compared to single-cell suspensions, cell aggregates retain superior cell viability, mimic in-vivo microenvironments and enhance functional properties, such as superior anti-inflammatory properties of mesenchymal stem/stromal cell aggregates or enhanced albumin production by hepatocyte aggregates. Cell aggregates can be either prepared by conventional spontaneous aggregation, or advanced substrate-based and technology-assisted methods, which have been well described in previous reviews. However, hydrogel-based preparation of cell aggregates is a novel, hitherto rarely considered method compared to conventional preparation of cell aggregates, such as hanging drops and commercial AggreWell™ plates. This review will mainly focus on two-dimensional (2D) hydrogel surface -based and three-dimensional (3D) hydrogel embedding-based preparation of cell aggregates by revealing the underlying mechanisms and highlighting some of their potential biomedical applications.
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© 2020 Elsevier Ltd. All rights reserved.