Hydrogel-based preparation of cell aggregates for biomedical applications
| dc.contributor.author | Zhang, J. | |
| dc.contributor.author | Yun, S. | |
| dc.contributor.author | Du, Y. | |
| dc.contributor.author | Zannettino, A. | |
| dc.contributor.author | Zhang, H. | |
| dc.date.issued | 2020 | |
| dc.description.abstract | Cell aggregates are widely used either as in-vitro models for drug screening, fundamental studies on disease progression and developmental biology, as well as in-vivo injectable cells for tissue regeneration. Compared to single-cell suspensions, cell aggregates retain superior cell viability, mimic in-vivo microenvironments and enhance functional properties, such as superior anti-inflammatory properties of mesenchymal stem/stromal cell aggregates or enhanced albumin production by hepatocyte aggregates. Cell aggregates can be either prepared by conventional spontaneous aggregation, or advanced substrate-based and technology-assisted methods, which have been well described in previous reviews. However, hydrogel-based preparation of cell aggregates is a novel, hitherto rarely considered method compared to conventional preparation of cell aggregates, such as hanging drops and commercial AggreWell™ plates. This review will mainly focus on two-dimensional (2D) hydrogel surface -based and three-dimensional (3D) hydrogel embedding-based preparation of cell aggregates by revealing the underlying mechanisms and highlighting some of their potential biomedical applications. | |
| dc.description.statementofresponsibility | Jiabin Zhang, Seonho Yun, Yuguang Du, Andrew Zannettino, Hu Zhang | |
| dc.identifier.citation | Applied Materials Today, 2020; 20:100747-1-100747-19 | |
| dc.identifier.doi | 10.1016/j.apmt.2020.100747 | |
| dc.identifier.issn | 2352-9407 | |
| dc.identifier.issn | 2352-9407 | |
| dc.identifier.orcid | Zannettino, A. [0000-0002-6646-6167] | |
| dc.identifier.orcid | Zhang, H. [0000-0003-4178-6401] | |
| dc.identifier.uri | http://hdl.handle.net/2440/128772 | |
| dc.language.iso | en | |
| dc.publisher | Elsevier | |
| dc.relation.grant | http://purl.org/au-research/grants/arc/DP160104632 | |
| dc.rights | © 2020 Elsevier Ltd. All rights reserved. | |
| dc.source.uri | https://doi.org/10.1016/j.apmt.2020.100747 | |
| dc.subject | 2D; 3D; hydrogel; cell aggregate; biomedical application | |
| dc.title | Hydrogel-based preparation of cell aggregates for biomedical applications | |
| dc.type | Journal article | |
| pubs.publication-status | Published |