Cytokine production by human milk cells and peripheral blood mononuclear cells from the same mothers
| dc.contributor.author | Hawkes, J. | |
| dc.contributor.author | Bryan, D. | |
| dc.contributor.author | Gibson, R. | |
| dc.date.issued | 2002 | |
| dc.description.abstract | Samples of milk (n=80) and venous blood were collected at 5 weeks postpartum from 82 lactating mothers. Human milk cells and peripheral blood mononuclear cells were isolated and the production of interleukin-1β, interleukin-6, and tumor necrosis factor-α in the absence and presence of lipopolysaccharide was determined by enzyme-linked immunosorbent assay. Human milk cells spontaneously produced significantly less interleukin-1β, interleukin-6, and tumor necrosis factor-α than peripheral blood mononuclear cells in the absence of stimulation. In vitro stimulation of human milk cells with lipopolysaccharide (500 ng/ml) for 24 hr increased cytokine production by approximately 40–50%, whereas peripheral blood mononuclear cells responded to lipopolysaccharide (200 ng/ml) with increased cytokine production of up to 350%. These observations suggest that cells in milk are capable of active involvement in the production of the interleukin-1β, interleukin-6, and tumor necrosis factor-α in the mammary gland and have the capacity to respond to further stimulation after leaving the breast. | |
| dc.description.statementofresponsibility | Joanna S. Hawkes, Dani-Louise Bryan and Robert A. Gibson | |
| dc.identifier.citation | Journal of Clinical Immunology, 2002; 22(6):338-344 | |
| dc.identifier.doi | 10.1023/A:1020652215048 | |
| dc.identifier.issn | 0271-9142 | |
| dc.identifier.issn | 1573-2592 | |
| dc.identifier.orcid | Gibson, R. [0000-0002-8750-525X] | |
| dc.identifier.uri | http://hdl.handle.net/2440/7463 | |
| dc.language.iso | en | |
| dc.publisher | Kluwer Academic/plenum Publ | |
| dc.rights | © Springer, Part of Springer Science+Business Media | |
| dc.source.uri | https://doi.org/10.1023/a:1020652215048 | |
| dc.subject | Human milk | |
| dc.subject | human milk cells | |
| dc.subject | peripheral blood mononuclear cells | |
| dc.subject | interleukin-1β | |
| dc.subject | interleukin-6 | |
| dc.subject | tumor necrosis factor-α | |
| dc.title | Cytokine production by human milk cells and peripheral blood mononuclear cells from the same mothers | |
| dc.type | Journal article | |
| pubs.publication-status | Published |