A novel method for detecting antigen-specific human regulatory T cells
Date
2012
Authors
Ebert, L.M.
MacRaild, S.E.
Davis, I.D.
Cebon, J.
Chen, W.
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Journal article
Citation
Journal of Immunological Methods, 2012; 377(1-2):56-61
Statement of Responsibility
Lisa M.Ebert, Sarah E.MacRaild, Ian D.Davis, Jonathan Cebon, Weisan Chen
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Abstract
Antigenic epitopes recognized by FoxP3(+) regulatory T cells (Treg) are poorly defined, largely due to a lack of assays for determining Treg specificity. We have developed a novel approach for detecting human Treg specific to peptide antigen, utilizing down-regulation of surface CD3 as a read-out of antigen recognition. Culture conditions and re-stimulation time have been optimized, allowing the detection of even very rare Treg, such as those specific to tumor antigens.
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© 2012 Elsevier B.V. All rights reserved.