A novel method for detecting antigen-specific human regulatory T cells

Simple item page
dc.contributor.authorEbert, L.M.
dc.contributor.authorMacRaild, S.E.
dc.contributor.authorDavis, I.D.
dc.contributor.authorCebon, J.
dc.contributor.authorChen, W.
dc.date.issued2012
dc.description.abstractAntigenic epitopes recognized by FoxP3(+) regulatory T cells (Treg) are poorly defined, largely due to a lack of assays for determining Treg specificity. We have developed a novel approach for detecting human Treg specific to peptide antigen, utilizing down-regulation of surface CD3 as a read-out of antigen recognition. Culture conditions and re-stimulation time have been optimized, allowing the detection of even very rare Treg, such as those specific to tumor antigens.
dc.description.statementofresponsibilityLisa M.Ebert, Sarah E.MacRaild, Ian D.Davis, Jonathan Cebon, Weisan Chen
dc.identifier.citationJournal of Immunological Methods, 2012; 377(1-2):56-61
dc.identifier.doi10.1016/j.jim.2012.01.004
dc.identifier.issn0022-1759
dc.identifier.issn1872-7905
dc.identifier.orcidEbert, L.M. [0000-0002-8041-9666]
dc.identifier.urihttp://hdl.handle.net/2440/128383
dc.language.isoen
dc.publisherElsevier
dc.relation.grantNHMRC
dc.rights© 2012 Elsevier B.V. All rights reserved.
dc.source.urihttps://doi.org/10.1016/j.jim.2012.01.004
dc.subjectFox P3, Antigen-specific regulatory T cells; CD3 down-regulation; NY-ESO-1
dc.titleA novel method for detecting antigen-specific human regulatory T cells
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Biochemistry publications