Intraduodenal calcium enhances the effects of L-tryptophan to stimulate gut hormone secretion and suppress energy intake in healthy males: a randomized, cross-over, clinical trial

Simple item page
dc.contributor.authorAnjom-Shoae, J.
dc.contributor.authorFitzgerald, P.C.
dc.contributor.authorHorowitz, M.
dc.contributor.authorMohammadpour, Z.
dc.contributor.authorHall, G.V.
dc.contributor.authorHolst, J.J.
dc.contributor.authorRehfeld, J.F.
dc.contributor.authorVeedfald, S.
dc.contributor.authorFeinle-Bisset, C.
dc.date.issued2024
dc.description.abstractBackground: In humans, intraduodenal infusion of L-tryptophan (Trp) increases plasma concentrations of gastrointestinal hormones and stimulates pyloric pressures, both key determinants of gastric emptying and associated with potent suppression of energy intake. The stimulation of gastrointestinal hormones by Trp has been shown, in preclinical studies, to be enhanced by extracellular calcium and mediated in part by the calcium-sensing receptor. Objectives: This study aim was to determine whether intraduodenal calcium can enhance the effects of Trp to stimulate gastrointestinal hormones and pyloric pressures and, if so, whether it is associated with greater suppression of energy intake, in healthy males. Methods: Fifteen males with normal weight (mean ± standard deviation; age: 26 ± 7 years; body mass index: 22 ± 2 kg/m2), received on 3 separate occasions, 150-min intraduodenal infusions of 0, 500, or 1000 mg calcium (Ca), each combined with Trp (load: 0.1 kcal/min, with submaximal energy intake-suppressant effects) from t = 75–150 min, in a randomized, double-blind, crossover study. Plasma concentrations of GI hormones [gastrin, cholecystokinin, glucose-dependent insulinotropic polypeptide (GIP), glucagon-like peptide (GLP)-1, and peptide tyrosine-tyrosine (PYY)], and Trp and antropyloroduodenal pressures were measured throughout. Immediately postinfusions (t = 150–180 min), energy intake at a standardized buffet-style meal was quantified. Results: In response to calcium alone, both 500- and 1000-mg doses stimulated PYY, while only the 1000-mg dose stimulated GLP-1 and pyloric pressures (all P < 0.05). The 1000-mg dose also enhanced the effects of Trp to stimulate cholecystokinin and GLP-1, and both doses stimulated PYY but, surprisingly, reduced the stimulation of GIP (all P < 0.05). Both doses substantially and dose dependently enhanced the effects of Trp to suppress energy intake (Ca-0+Trp: 1108 ± 70 kcal; Ca-500+Trp: 961 ± 90 kcal; and Ca-1000+Trp: 922 ± 96 kcal; P < 0.05). Conclusions: Intraduodenal administration of calcium enhances the effect of Trp to stimulate plasma cholecystokinin, GLP-1, and PYY and suppress energy intake in healthy males. These findings have potential implications for novel nutrient-based approaches to energy intake regulation in obesity.
dc.description.statementofresponsibilityJavad Anjom-Shoae, Penelope CE Fitzgerald, Michael Horowitz, Zinat Mohammadpour, Gerrit van Hall, Jens J Holst, Jens F Rehfeld, Simon Veedfald, Christine Feinle-Bisset
dc.identifier.citationAmerican Journal of Clinical Nutrition, 2024; 120(3):528-539
dc.identifier.doi10.1016/j.ajcnut.2024.07.006
dc.identifier.issn0002-9165
dc.identifier.issn0002-9165
dc.identifier.orcidFitzgerald, P.C. [0000-0002-2775-9776]
dc.identifier.orcidHorowitz, M. [0000-0002-0942-0306]
dc.identifier.orcidFeinle-Bisset, C. [0000-0001-6848-0125]
dc.identifier.urihttps://hdl.handle.net/2440/143650
dc.language.isoen
dc.publisherElsevier
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1103020
dc.rights© 2024 American Society for Nutrition. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
dc.source.urihttp://dx.doi.org/10.1016/j.ajcnut.2024.07.006
dc.subjectAmino acid
dc.subjectcalcium
dc.subjectcholecystokinin
dc.subjectfood intake
dc.subjectglucagon-like peptide-1
dc.subjectpeptide tyrosine tyrosine
dc.subject.meshDuodenum
dc.subject.meshHumans
dc.subject.meshCalcium
dc.subject.meshGastrointestinal Hormones
dc.subject.meshCholecystokinin
dc.subject.meshPeptide YY
dc.subject.meshTryptophan
dc.subject.meshCross-Over Studies
dc.subject.meshDouble-Blind Method
dc.subject.meshEnergy Intake
dc.subject.meshGastric Emptying
dc.subject.meshAdult
dc.subject.meshMale
dc.subject.meshGlucagon-Like Peptide 1
dc.subject.meshYoung Adult
dc.titleIntraduodenal calcium enhances the effects of L-tryptophan to stimulate gut hormone secretion and suppress energy intake in healthy males: a randomized, cross-over, clinical trial
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Research Outputs