Oral and parenteral immunization with synthetic retro-inverso peptides induce antibodies that cross-react with native peptides and parent antigens
Date
2007
Authors
Fischer, Peter
Comis, Alfio
Tyler, Margaret
Howden, Merlin
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Indian Journal of Biochemistry and Biophysics, 2007; 44 (3):140-144
Statement of Responsibility
Peter Fischer, Alfio Comis, Margaret Tyler and Merlin Howden
Conference Name
Abstract
The objective of this study was to determine whether certain retro-inverso peptides have the potential to act as synthetic vaccines in mice, when immunized by injection or orally. Immunization of mice parenterally with conjugates of three such retro-inverso peptides and orally with the unconjugated peptides elicited generally high titres of anti-peptide antibodies. Antibodies against the same three peptides cross-reacted by binding strongly in ELISA to the native peptides and vice versa, regardless of the mode of immunization. Antibodies against a retro-inverso diphtheria peptide also reacted strongly with diphtheria toxin. Seven of 8 mice, immunized by injection of the conjugate of a retro-inverso derivative of robustoxin [a lethal spider (Atrax robustus) venom toxin] were protected from challenge involving injection with twice the minimum lethal dose of A. robustus venom containing the toxin.
School/Discipline
School of Molecular and Biomedical Science